Calcium involvement in aminophospholipid exposure and microparticle formation during platelet activation: a study using Ca2+-ATPase inhibitors

Biochemistry
J Dachary-PrigentA T Nurden

Abstract

The development of procoagulant activity and microparticle formation during platelet activation is known to depend on an increase in cytosolic Ca2+ levels. We have studied the mechanisms leading to these events using FITC-labeled recombinant annexin V, a protein which binds with a high affinity to aminophospholipids, in flow cytometry. In particular, we show that the Ca(2+)-ATPase inhibitors thapsigargin and cyclopiazonic acid are as potent inducers of aminophospholipid exposure and microparticle formation as the ionophore A23187. In contrast, 2,5-di-tert-butyl-1, 4-benzohydroquinone induced negligible microparticle formation, although platelets abundantly bound annexin V-FITC. That platelet activation had occurred was confirmed by binding studies with VH10, a monoclonal antibody specific for the alpha-granule membrane glycoprotein GMP-140, and by prothrombinase activity measurements. These results demonstrate that microvesiculation is not an automatic response to aminophospholipid exposure. The Ca(2+)-ATPase inhibitors induced different intracellular Ca2+ levels as measured using fluo-3 as a calcium dye. These were 10 +/- 4 microM (n = 11) for thapsigargin (3 microM), 19.6 +/- 2.2 microM (n = 8) for cyclopiazonic acid (100 mic...Continue Reading

Citations

Jul 14, 2010·The Journal of Membrane Biology·Sárka PerutkováAles Iglic
Feb 19, 2009·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·François RouzetDominique Le Guludec
Jun 15, 1996·Thrombosis Research·J M PasquetJ Dachary-Prigent
May 29, 2004·Biochimica Et Biophysica Acta·Robert F A ZwaalEdouard M Bevers
May 26, 2005·Bioorganic & Medicinal Chemistry·Roger G Hanshaw, Bradley D Smith
Apr 23, 2003·Experimental Cell Research·Olivier GasserJürg A Schifferli
Nov 7, 1998·Biochimica Et Biophysica Acta·R F ZwaalE M Bevers
Aug 14, 1999·Biochimica Et Biophysica Acta·E M BeversR F Zwaal
May 12, 2006·Organic & Biomolecular Chemistry·Kristy M DiVittorioBradley D Smith
Jun 22, 2000·The Biochemical Journal·A Zweifach
Oct 16, 1999·British Journal of Haematology·A ShcherbinaE Remold-O'Donnell
Jan 22, 2009·Médecine sciences : M/S·Loïc Doeuvre, Eduardo Angles-Cano
Mar 23, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Atul AggarwalDavid J Schneider
Aug 1, 1996·European Journal of Biochemistry·J M PasquetA T Nurden
May 25, 2002·Veterinary Clinical Pathology·Melinda J. Wilkerson, Wilma Shuman
Jan 31, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Noriko TamuraShinya Goto
Dec 19, 2000·Biochemical and Biophysical Research Communications·S MartinM C Martínez
Mar 27, 2002·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·J CorralV Vicente
Mar 5, 2005·Platelets·J Polasek
Sep 29, 2009·Critical Reviews in Biochemistry and Molecular Biology·Bengt Fadeel, Ding Xue
Jan 31, 2012·The Veterinary Clinics of North America. Small Animal Practice·Pete W ChristophersonMary K Boudreaux
Jul 11, 2006·Microbes and Infection·Nicolas ColtelGeorges E Grau
Jul 31, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Atul AggarwalDavid J Schneider
Oct 1, 2004·Journal of Thrombosis and Haemostasis : JTH·J E PhillipsG E Gilbert

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