Calcium ion-dependent entry of the membrane-containing bacteriophage PM2 into its Pseudoalteromonas host

Virology
Virginija Cvirkaitė-KrupovičDennis H Bamford

Abstract

Marine bacteriophage PM2 infects gram-negative Pseudoalteromonas species and is currently the only assigned member of the Corticoviridae family. The icosahedral protein shell covers an internal protein-rich phage membrane that encloses the highly supercoiled dsDNA genome. In this study we investigated PM2 entry into the host. Our results indicate that PM2 adsorption to the host is dependent on the intracellular ATP concentration, while genome penetration through the cytoplasmic membrane depends on the presence of millimolar concentrations of calcium ions in the medium. In the absence of Ca(2+) the infection is arrested at the entry stage but can be rescued by the addition of Ca(2+). Interestingly, PM2 entry induces abrupt cell lysis if the host outer membrane is not stabilized by divalent cations. Experimental data described in this study in combination with results obtained previously allowed us to propose a sequential model describing the entry of bacteriophage PM2 into the host cells.

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Citations

Oct 4, 2013·Journal of Virology·Emmanuelle R J QueminMart Krupovic
Jun 15, 2013·Chemical Biology & Drug Design·Simona SutkuvienėRimantas Daugelavičius
Oct 11, 2012·Archaea : an International Microbiological Journal·Elina Roine, Dennis H Bamford
Mar 7, 2015·International Journal of Food Microbiology·Jennifer MahonyDouwe van Sinderen
Dec 14, 2011·Virology·Lina JakutytėRimantas Daugelavičius
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·Mart KrupovicDennis H Bamford
Feb 7, 2013·Nature Reviews. Microbiology·Ian J Molineux, Debabrata Panja
Mar 11, 2019·Journal of Dairy Science·X ChenB Munguntsetseg
Jan 4, 2020·Bioorganic & Medicinal Chemistry·Zijian ZhangShiguo Sun
Apr 4, 2017·Poultry Science·Ar'Quette GrantBob Vimini

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