PMID: 6160873Nov 11, 1980Paper

Calcium ion stimulated endogenous protein kinase catalyzed phosphorylation of basic proteins in myelin subfractions and myelin-like membrane fraction from rat brain

Biochemistry
P V SulakheB J Thiessen

Abstract

Polypeptide composition and endogenous phosphorylation were investigated in the subfractions of rat brain myelin isolated by either discontinuous or continuous sucrose density gradient centrifugation of myelin. Similarly, a myelin-like membrane fraction (SN4) was also studied. Observations were made that strongly indicated the presence of a calcium-stimulated protein kinase in a highly purified myelin preparation and which exclusively phosphorylated myelin basic proteins of the membrane preparation. Adenosine cyclic 3',5'-phosphate (cAMP) stimulated kinase on the other hand was found to be considerably enriched in the myelin-like membrane fraction. Although this latter enzyme is also capable of phosphorylating the basic proteins, its effect was at least 5 times weaker compared to the calcium-stimulated myelin protein kinase. Within the gradient subfractions there appeared a close relation between the amount of basic proteins and their calcium-stimulated phosphorylation; a similar relationship, however, was not obtained in the case of cAMP-dependent phosphorylation of myelin basic proteins. The former (i.e., Ca2+-stimulated phosphorylation) was found to require a protein factor that functionally resembled calmodulin. The results...Continue Reading

References

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Citations

Jan 1, 1984·Neurochemical Research·V ZabrenetzkyP S Spencer
Jan 1, 1988·Molecular Neurobiology·A T Campagnoni, W B Macklin
Jan 1, 1980·The International Journal of Biochemistry·E H Petrali, P V Sulakhe
Apr 1, 1985·Journal of the Neurological Sciences·J L NussbaumP Jollès
Jan 1, 1988·Progress in Neurobiology·J B Ulmer
Aug 1, 1983·The Journal of Cell Biology·K C DesJardins, P Morell
Oct 1, 1984·Neurochemical Research·N Kerlero De RosboD S Linthicum
Jul 14, 1989·Biochemical and Biophysical Research Communications·C Boulias, M A Moscarello
Aug 1, 1982·Journal of Neurochemistry·G I KristjanssonW H Gispen
Jul 1, 1984·Journal of Neurochemistry·N Murray, A J Steck
Feb 13, 1986·Biochemical and Biophysical Research Communications·K F ChanK P Huang
Oct 13, 1983·Nature·E J Nestler, P Greengard
Aug 14, 1987·Biochemical and Biophysical Research Communications·J B Ulmer, P E Braun
Jul 18, 1986·Science·Y Nishizuka

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