PMID: 2105886Feb 14, 1990Paper

Calcium-ionophore-induced formation of platelet-activating factor and leukotrienes by horse eosinophils: a comparative study

European Journal of Biochemistry
R Asmis, A Jörg

Abstract

Horse eosinophils preincubated with 3H-labelled acetate and stimulated with the Ca2+ ionophores ionomycin or A23187 form a radioactive compound, which we have shown to be 1-O-alkyl-2-[3H]acetyl-sn-glycero-3-phosphocholine (platelet-activating factor). We could detect no 1-O-acyl-2-[3H]acetyl-glycero-3-phosphocholine in the radioactive fraction. The formation of platelet-activating factor was strongly correlated to the generation of leukotriene C4, the main arachidonate metabolite in horse eosinophils, suggesting that platelet-activating factor and leukotriene C4 have a common precursor pool (1-O-alkyl-2-arachidonyl-glycero-3-phosphocholine) and a common regulation of synthesis. Even though both ionomycin and A23187 are described as Ca2+ ionophores, they have a series of significantly different effects on the eosinophils with respect to formation of platelet-activating factor and leukotriene C4. While A23187 induces an asymptotic maximum of mediator formation at concentrations higher than 20 microM, ionomycin expressed a narrow optimum at 2 microM. The effects of exogenous pH on the release of mediators also differ strongly between the two ionophores: for A23187 the effects are the same for both mediators but when ionomycin is u...Continue Reading

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Citations

Jan 1, 1995·Veterinary Immunology and Immunopathology·A P FosterF M Cunningham
May 1, 1997·Veterinary Immunology and Immunopathology·A P FosterF M Cunningham
May 1, 1992·Equine Veterinary Journal·A P FosterP Lees
Apr 2, 2003·Journal of Veterinary Pharmacology and Therapeutics·A P Foster, F M Cunningham
Mar 26, 2005·Prostaglandins & Other Lipid Mediators·Eric GaudreaultMarek Rola-Pleszczynski
Oct 1, 1992·The Journal of Allergy and Clinical Immunology·U R JuergensB L Zuraw
Aug 15, 2000·Journal of Chromatography. a·V Ruiz-Gutiérrez, M C Pérez-Camino

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