Calcium is required for the expression of anthrax lethal toxin activity in the macrophagelike cell line J774A.1.

Infection and Immunity
Rakesh BhatnagarA Friedlander

Abstract

Anthrax lethal toxin, which consists of two separate proteins, protective antigen (Mr, 82,700) and lethal factor (Mr, approximately 83,000), is cytotoxic to the macrophagelike cell line J774A.1. Removal of calcium from the culture medium protected cells against the action of lethal toxin. Calcium depletion during the binding phase of intoxication afforded only partial protection. Further analysis showed that calcium removal caused some inhibition of protective antigen binding but that it had minimal effect on proteolytic conversion of protective antigen to the active 63-kilodalton fragment and that it had no effect on lethal factor binding. Cells to which lethal toxin had bound in the presence of calcium were protected when transferred to calcium-depleted culture medium, indicating a role for calcium at a postbinding stage. When ammonium chloride is present with lethal toxin, toxin accumulates in intracellular vesicles. Calcium-free medium protected these cells upon removal of the amine block, suggesting that calcium is also required at a step after internalization of lethal toxin. Calcium channel blockers inhibited 45Ca2+ uptake and protected cells against cytotoxicity. Calmodulin inhibitors also protected against the action o...Continue Reading

References

Apr 14, 1978·Biochemical and Biophysical Research Communications·A H TashjianD Maina
Jun 13, 1979·Biochemical and Biophysical Research Communications·R KobayashiH Hidaka
Nov 9, 1979·Science·F A SchanneJ L Farber
Jan 1, 1988·Cancer Treatment and Research·S Olsnes, K Sandvig
Jan 1, 1988·Methods in Enzymology·S H Leppla
Sep 1, 1988·Archives of Biochemistry and Biophysics·J O Tsokos-Kuhn
Jan 1, 1986·European Neurology·B K Siesjö
Feb 1, 1987·Infection and Immunity·K Sandvig, J E Brown
Oct 31, 1986·Biochimica Et Biophysica Acta·Y HoriguchiG Sakaguchi
Dec 1, 1981·The Journal of Cell Biology·S B HorwitzO M Rosen
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S H Leppla
Jan 1, 1984·Medicinal Research Reviews·J R Guerrero, S S Martin
Dec 1, 1983·Microbiological Reviews·L EidelsD A Hart
Sep 1, 1984·Microbiological Reviews·J L Middlebrook, R B Dorland
Apr 1, 1984·Journal of Cellular Physiology·A SundanS Olsnes
Feb 1, 1982·Infection and Immunity·D FitzgeraldC B Saelinger
Sep 28, 1981·Life Sciences·J L Farber
Mar 1, 1980·The American Journal of Physiology·C Carter-Su, G A Kimmich

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Citations

May 15, 2002·FEMS Microbiology Letters·Aparna SinghRakesh Bhatnagar
Jul 12, 2007·The Journal of Biological Chemistry·Soon-Duck HaSung Ouk Kim
Jan 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xingjian XuDavid J Weber
Feb 23, 2007·The Biochemical Journal·Benjamin E Turk
Mar 23, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Andrew W Artenstein, Steven M Opal
Aug 17, 2002·Infection and Immunity·Praveen KumarRakesh Bhatnagar
Mar 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Susan L FinkBrad T Cookson
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jeanette I WebsterEsther M Sternberg
Sep 22, 2010·Infection and Immunity·Robert E Bolcome, Joanne Chan
Dec 15, 1994·FEMS Microbiology Letters·S K KochiC Montecucco
Feb 7, 1998·Infection and Immunity·P GuptaR Bhatnagar
Apr 4, 2021·International Journal of Molecular Sciences·Dinendra L AbeyawardhaneDavid J Weber
Jul 27, 2001·Biochemical and Biophysical Research Communications·P GuptaR Bhatnagar
Feb 13, 2001·Biochemical and Biophysical Research Communications·P GuptaR Bhatnagar
May 1, 2001·Biochemical and Biophysical Research Communications·V ChauhanR Bhatnagar
Sep 14, 2001·Biochemical and Biophysical Research Communications·N AhujaR Bhatnagar
Jun 9, 2001·Biochemical and Biophysical Research Communications·P GuptaR Bhatnagar
Dec 14, 1999·Microbes and Infection·S F Little, B E Ivins
Jul 1, 1994·Infection and Immunity·R Bhatnagar, A M Friedlander
Jan 1, 1993·Infection and Immunity·A M FriedlanderY Singh
Jul 26, 2003·Protein Expression and Purification·Joungmok KimMoon-Young Yoon
Dec 16, 2003·Biochemical and Biophysical Research Communications·Joungmok KimMoon-Young Yoon
May 1, 2004·Trends in Microbiology·Ana M Sánchez, Kenneth A Bradley
Jul 30, 2009·Molecular Aspects of Medicine·Mahtab Moayeri, Stephen H Leppla

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