PMID: 7015328Feb 1, 1981Paper

Calcium lability of cytoplasmic microtubules and its modulation by microtubule-associated proteins

Proceedings of the National Academy of Sciences of the United States of America
M SchliwaJ G Izant

Abstract

Detergent-extracted BSC-1 monkey cells have been used as a model system to study the Ca(2+) sensitivity of in vivo polymerized microtubules under in vitro conditions. The effects of various experimental treatments were observed by immunofluorescence microscopy. Whereas microtubules are completely stable at Ca(2+) concentrations below 1 muM, Ca(2+) at greater than 1-4 muM induces microtubule disassembly that begins in the cell periphery and proceeds towards the cell center. At concentrations of up to 500 muM, both the pattern and time course of disassembly are not markedly altered, suggesting that, within this concentration range, Ca(2+) effects are catalytic rather than stoichiometric. Higher (millimolar) Ca(2+) concentration results in rapid destruction of microtubules. Of other divalent cations, only Sr(2+) has a slight depolymerizing effect, whereas millimolar Ba(2+), Mg(2+), or Mn(2+) is ineffective. Disassembly induced by micromolar Ca(2+) is inhibited by pharmacological agents known to bind to calmodulin and inhibit its function, suggesting that calmodulin mediates Ca(2+) effects. Both the addition of exogenous brain microtubule-associated proteins (MAPs) after lysis and the retention of endogenous cellular MAPs normally ...Continue Reading

References

Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J M MarcumA R Means
Jul 1, 1975·Biochemistry·J B Olmsted, G G Borisy
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M J WelshA R Means
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·K WeberM Osborn
Jan 1, 1976·Journal of Supramolecular Structure·G M Fuller, B R Brinkley
Feb 8, 1977·Biochemistry·F Solomon
May 8, 1980·Nature·A R Means, J R Dedman
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J G Izant, J R McIntosh

❮ Previous
Next ❯

Citations

Jan 1, 1994·Cell Motility and the Cytoskeleton·S J Chapin, J C Bulinski
Sep 1, 1991·Journal of Neuroscience Research·M E Dailey, P C Bridgman
Apr 1, 1984·The Histochemical Journal·J PalecekJ Nedvídek
Apr 1, 1990·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J OchiengJ Russo
Oct 1, 1988·Developmental Biology·B MaroM Paintrand
Sep 1, 1989·Developmental Biology·J K Ivins, R N Pittman
Jan 1, 1985·The International Journal of Biochemistry·J E Hesketh
Jan 1, 1984·Pharmacology & Therapeutics·J J Manfredi, S B Horwitz
Nov 1, 1990·Brain Research. Developmental Brain Research·P E SpoerriF J Roisen
Aug 12, 1994·Brain Research. Developmental Brain Research·D K Williams, C S Cohan
Jul 20, 1982·Neuroscience Letters·R D BurgoyneJ Barron
Mar 1, 1982·Neuroscience·P R Gordon-WeeksE G Gray
Jan 1, 1990·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·P Dubovy, P Haninec
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B A Perrino, I N Chou
Sep 30, 1986·Biochemical and Biophysical Research Communications·B D Clark, I R Brown
Sep 21, 1990·Journal of Theoretical Biology·M A Chaplain, B D Sleeman
May 10, 2003·Life Sciences·Qianli YuRonald R Watson
Dec 1, 2001·Progress in Neurobiology·S D SkaperF S Walsh
Jun 26, 1999·The European Journal of Neuroscience·B CalabreseM Pellegrino
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SchliwaK R Porter
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·D R WebsterG G Borisy
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K SchützeM Schliwa
Nov 11, 2010·The Journal of Biological Chemistry·Julien LefèvrePhilippe Savarin
Oct 1, 1985·The Journal of Cell Biology·K Luby-PhelpsD L Taylor
Nov 1, 1985·The Journal of Cell Biology·B MaroM Webb
Jul 1, 1987·The Journal of Cell Biology·D R WebsterG G Borisy
Sep 1, 1989·The Journal of Cell Biology·K L Lankford, P C Letourneau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.