Calcium mobilization and response recovery following P2-purinoceptor stimulation of rat isolated alveolar type II cells

British Journal of Pharmacology
C C DornF M Singleton

Abstract

1. The effect of adenosine 5'-triphosphate (ATP) on surfactant phospholipid secretion, calcium mobilization, and the time course for recovery of the response system was studied in isolated alveolar Type II cells of the rat. 2. ATP (10 microM) stimulated a biphasic intracellular Ca2+ transient monitored by changes in Fura-2 fluorescence, from a basal level of 126 +/- 9 nM, to a rapid peak of 391 +/- 1 nM, followed by a prolonged plateau 26 +/- 4 nM above baseline (mean +/- s.e.mean, n = 26). 3. ATP-stimulated surfactant phospholipid secretion and peak Ca2+ levels had similar EC50s (1 x 10(-6) M), and were unaffected by chelation of extracellular Ca2+. However, the prolonged plateau phase was abolished by chelation of extracellular Ca2+. 4. There was a 15 min refractory period before full recovery of the Ca2+-response to ATP. Recovery was dependent on extracellular Ca2+, was accelerated by removing extracellular agonist and was prolonged following stimulation with the poorly hydrolyzed ATP analogue, ATP-gamma-S. 5. While the Type II cell was capable of multiple ATP-induced Ca2+ transients following recovery, no additional surfactant phospholipid was released with sequential stimulation. 6. These findings suggest initial exposure ...Continue Reading

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Citations

May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T A Voyno-YasenetskayaR L Hamilton
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·T HallerP Dietl
Jul 26, 2002·Mediators of Inflammation·Georg HoffmannRainer Meyer
Feb 16, 2005·Annual Review of Physiology·Paul Dietl, Thomas Haller
Oct 6, 2001·American Journal of Respiratory Cell and Molecular Biology·M FrickP Dietl
Jun 20, 2006·Free Radical Biology & Medicine·Shama AhmadCarl W White
May 16, 2006·Journal of Biomolecular Screening·A WemhönerT Haller
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Michael Koval
Feb 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·B E IsaksonS Boitano
Sep 8, 2001·American Journal of Physiology. Cell Physiology·B E IsaksonS Boitano
May 18, 1999·The American Journal of Physiology·E MatthewJ Dedman

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