Calcium mobilization via sphingosine kinase in signalling by the Fc epsilon RI antigen receptor

Nature
O H ChoiJ P Kinet

Abstract

Calcium mobilization through antigen receptors, including high-affinity IgE receptors (Fc epsilon RI), is thought to be mediated by inositol-1,4,5-trisphosphate production (InsP3). Here we show that antigen clustering of Fc epsilon RI on the rat mast-cell line (RBL-2H3) activates a sphingosine kinase (SK) and produces sphingosine-1-phosphate (S1P), and alternative second messenger for intracellular calcium mobilization. The sphingosine analogue, D-L-threo-dihydrosphingosine (DHS), inhibits the SK enzyme competitively with a dissociation constant, K1, of 5 to 18 microM. This inhibition substantially suppresses the Fc epsilon RI-mediated calcium signal, but leaves intact the syk tyrosine kinase activation and the small InsP3 production. The entire InsP3-dependent pathway activated by a transfected G-protein coupled receptor, used here as a positive control, also remained intact. Thus Fc epsilon RI principally utilizes a SK pathway to mobilize calcium.

References

May 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W StoffelB Hellenbroich
Oct 1, 1973·Hoppe-Seyler's Zeitschrift für physiologische Chemie·W StoffelG Heimann
Jul 16, 1993·Cell·W KolanusB Seed
Jan 28, 1993·Nature·M J Berridge
May 1, 1993·Immunology Today·M A Beaven, H Metzger

❮ Previous
Next ❯

Citations

Jan 6, 1999·Archives of Pharmacal Research·H CheongK M Kim
Apr 13, 2010·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Eun-Ju LeeMi-Kyung Sung
Aug 2, 2011·Seminars in Immunopathology·Hideru Obinata, Timothy Hla
Mar 8, 2011·Archives of Pharmacal Research·Jeong Ho JinHyun Pyo Kim
Jan 20, 2004·Biochimica Et Biophysica Acta·Yusuke NakadeTakashi Murate
Aug 29, 2003·Biochemical and Biophysical Research Communications·Yu FukudaYasuyuki Igarashi
Aug 15, 2000·European Journal of Pharmacology·K W YoungS R Nahorski
Mar 10, 2001·European Journal of Pharmacology·D Meyer zu HeringdorfK H Jakobs
Sep 3, 1998·European Journal of Pharmacology·D Meyer zu HeringdorfK H Jakobs
Jun 4, 1999·Progress in Biophysics and Molecular Biology·M HuberG Krystal
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·Y YatomiY Igarashi
Apr 28, 2001·Prostaglandins & Other Lipid Mediators·A Olivera, S Spiegel
Sep 10, 2002·Molecular Immunology·Puneet S JollySarah Spiegel
Nov 30, 2000·Immunology Today·E E Prieschl, T Baumruker
Jul 20, 1999·Molecular and Cellular Endocrinology·L T BudnikA K Mukhopadhyay
Dec 1, 1996·Current Opinion in Immunology·M A Beaven, R A Baumgartner
Feb 1, 1997·Current Opinion in Immunology·J V Ravetch
Apr 1, 1997·Current Opinion in Cell Biology·L Y Jan, Y N Jan
Jan 1, 1999·Bioorganic & Medicinal Chemistry Letters·L QiaoS Spiegel
Apr 5, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Benedetta CinqueM Grazia Cifone
Apr 13, 2000·Biochimica Et Biophysica Acta·S Spiegel, S Milstien
Jun 19, 2002·Biochimica Et Biophysica Acta·Susan Pyne, Nigel J Pyne
Jul 7, 2005·Nature Reviews. Immunology·Hugh Rosen, Edward J Goetzl
Feb 14, 2006·Nature Reviews. Immunology·Alasdair M Gilfillan, Christine Tkaczyk
Apr 18, 2007·Nature Reviews. Immunology·Stefan Kraft, Jean-Pierre Kinet
Sep 13, 2008·Nature Reviews. Immunology·Juan RiveraAna Olivera
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Matthias P Wymann, Roger Schneiter
Jul 6, 2000·The Biochemical Journal·S Pyne, N J Pyne
Feb 21, 2002·The Biochemical Journal·Elisabetta MeacciPaola Bruni
Dec 3, 1999·British Journal of Haematology·L YangY Ozaki
Oct 23, 2002·Journal of Neurochemistry·Rosario GarridoMichael Mulholland
Jul 23, 2008·The Journal of Biological Chemistry·Nicholas J MacheskyJoanne Trgovcich
Apr 10, 1999·Molecular Biology of the Cell·C H LiuT Hla

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.