Calcium-mobilizing purine receptors on the surface of mammalian articular chondrocytes

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
M Koolpe, H P Benton

Abstract

If we are to fully understand mechanisms of cartilage homeostasis, it is essential that we know the full catalogue of receptors present on the surface of a chondrocyte and the pathways regulated by ligands that bind to these receptors. In this study, we describe chondrocyte responses to adenosine 5'-triphosphate and related molecules. Adenosine 5'-triphosphate stimulated a statistically significant, dose-dependent, transient rise in the concentration of calcium ions in Fura 2-loaded, differentiated, primary chondrocytes. The increase occurred in the absence of extracellular calcium, indicating a mobilization from intracellular stores. The increase in concentration of cytoplasmic calcium ions induced by adenosine 5'-triphosphate was mimicked by uridine 5'-triphosphate but not by 2-methylthioadenosine 5'-triphosphate, cytidine 5'-triphosphate, or adenosine. Heterologous desensitization experiments demonstrated that chondrocytes showed no subsequent response to uridine 5'-triphosphate after initial stimulation with adenosine 5'-triphosphate nor did they respond to adenosine 5'-triphosphate in inverse conditions, thereby indicating competition for the same receptor site. Together, these results are consistent with the presence of a...Continue Reading

References

Feb 19, 1992·Biochimica Et Biophysica Acta·C el-MoatassimJ C Mani
Nov 30, 1992·FEBS Letters·A P NaumovG N Mozhayeva
Oct 6, 1992·Biochimica Et Biophysica Acta·A M CaswellR G Russell
Jul 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H KumagaiC R Filburn
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K A HogquistD D Chaplin
Jan 1, 1990·Annals of the New York Academy of Sciences·G Burnstock
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P G GreenJ D Levine
Feb 1, 1986·Cell Calcium·J W Putney
Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Jan 15, 1986·The Biochemical Journal·J L Gordon
Jul 15, 1988·Biochemical and Biophysical Research Communications·H P Benton, J A Tyler
Jan 1, 1987·Annual Review of Biochemistry·M J Berridge
Sep 21, 1984·Science·M Schramm, Z Selinger
Jan 1, 1982·Connective Tissue Research·R W FarndaleA J Barrett
Jul 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·W B BowlerG Bilbe
Jul 17, 1995·Biochemical and Biophysical Research Communications·D J HendersonI A Dainty
Aug 24, 1995·Biochemical and Biophysical Research Communications·C SchöflG Brabant
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K D LustigD Julius
Mar 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·W A BulmanC C Clark
Feb 1, 1995·Cell Calcium·A D Conigrave, L Jiang
Jun 6, 1995·Biochemical and Biophysical Research Communications·Y TokuyamaG I Bell
Nov 11, 1994·Biochimica Et Biophysica Acta·W S LeongA M Caswell
Sep 1, 1994·Trends in Pharmacological Sciences·C Kennedy, A IJzerman
May 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S S StojilkovicF P Luyten
Sep 1, 1993·The American Journal of Physiology·G R Dubyak, C el-Moatassim

❮ Previous
Next ❯

Citations

Aug 15, 2013·Purinergic Signalling·Geoffrey BurnstockIsabel R Orriss
Jan 10, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Angus K T WannMartin M Knight
Mar 30, 2010·Acta Physiologica·G BurnstockA Verkhratsky
Oct 15, 2003·Arthritis and Rheumatism·Maryse PicherGreta M Lee
Nov 6, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Mariana Garcia, Martin M Knight
Aug 17, 2010·Osteoarthritis and Cartilage·A K RosenthalL M Ryan
May 17, 2015·Autonomic Neuroscience : Basic & Clinical·Isabel R Orriss
Aug 25, 2001·Osteoarthritis and Cartilage·M K ElfervigA J Banes
Dec 13, 2005·Cell Biochemistry and Function·Taisuke KonoKoh-ichi Enomoto
Aug 3, 1999·Journal of Cellular Physiology·C E YellowleyH J Donahue
Jul 21, 2000·Arthritis and Rheumatism·R D GraffG M Lee
Jun 11, 1998·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M KoolpeH P Benton

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.