PMID: 6406220May 16, 1983Paper

Calcium modulates fatty acid dynamics in rat liver plasma membranes

European Journal of Biochemistry
F Schroeder, C Soler-Argilaga

Abstract

Modulation of free fatty acid binding in isolated rat liver plasma membranes was evaluated using the fluorescent fatty acids trans-parinaric and cis-parinaric acid as analogues for saturated and unsaturated fatty acids, respectively. Binding of trans-parinarate but not cis-parinarate was inhibited by physiological levels of Ca2+. The effect was reversed by addition of excess EGTA. Calcium decreased the aqueous to lipid partition coefficient, Kp, of trans-parinaric acid for liver plasma membranes while increasing the Kp for cis-parinaric acid. In addition, Ca2+ also altered the fluorescence lifetime, the quantum yield, and the relative partitioning of trans-parinaric and cis-parinaric acid into fluid and solid phases. Calcium and EGTA did not affect the binding of 1,6-diphenyl-1,3,5-hexatriene. The effect of Ca2+ on the liver plasma membrane structure was to increase the rigidity of the membrane, primarily the solid domain. The fluorescence polarization of trans-parinarate, cis-parinarate, and 1,6-diphenyl-1,3,5-hexatriene at 24 degrees C in liver plasma membranes in the absence of Ca2+ was 0.295 +/- 0.008, 0.253 +/- 0.007, and 0.284 +/- 0.005, respectively. Calcium (2.4 mM) increased the polarization of these probe molecules in...Continue Reading

References

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Citations

Jun 1, 1984·Mechanisms of Ageing and Development·F SchroederE Roberts
May 1, 1986·Molecular and Cellular Endocrinology·G Deliconstantinos, S Fotiou
May 16, 1983·European Journal of Biochemistry·F Schroeder
Jul 2, 1984·European Journal of Biochemistry·F SchroederE Roberts
Aug 1, 1984·Journal of Neurochemistry·F SchroederE Roberts
Dec 1, 1991·Hypertension·A F DominiczakD F Bohr
May 20, 2004·The Biochemical Journal·Adalberto M GallegosFriedhelm Schroeder
Sep 3, 1987·Biochimica Et Biophysica Acta·F SchroederW G Wood
Oct 3, 1984·Biochimica Et Biophysica Acta·F Schroeder

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