PMID: 8948490Nov 14, 1996Paper

Calcium modulates plasmin cleavage of the fibrinogen D fragment gamma chain N-terminus: mapping of monoclonal antibody J88B to a plasmin sensitive domain of the gamma chain

Biochimica Et Biophysica Acta
T M OdrljinP J Simpson-Haidaris

Abstract

Plasmin sensitive sites are found on the A alpha, B beta and gamma chains of fibrinogen at regions joining the two C-terminal D fragments with the central E fragment. We have developed a monoclonal antibody (MoAb) reactive with this plasmin sensitive region on the human fibrinogen gamma chain and mapped its epitope. MoAb J88B reacts with gamma chains of both native as well as with reduced and denatured fibrinogen and fibrin, the CNBr fragment of the fibrinogen central domain, plasmin cleaved fragments D, gamma-gamma dimers, but not with plasmic fragments E. These data indicate that J88B maps to the plasmin sensitive domain localized to gamma 63-78. MoAb J88B failed to react with synthetic peptide gamma 70-78, which suggests that the epitope includes the newly exposed N-terminal residues gamma 63-70 of the early plasmic fragment D1A. As calcium has a marked influence on plasmin cleavage of C-terminal sites on the gamma chain, the effects of calcium on modulating plasmin cleavage of D1A to D1 were assessed in the absence or presence of J88B. The results indicated that calcium delays and J88B (+/- calcium) protects the gamma chain from plasmin cleavage at the N-terminus of D1A, suggesting that this enzymatically labile site is cal...Continue Reading

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Citations

Jan 12, 2001·Thrombosis Research·A E MichelsenF Brosstad
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A Sahni, C W Francis
Oct 24, 2007·Journal of Thrombosis and Haemostasis : JTH·A SahniC W Francis
Dec 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·T M OdrljinP J Simpson-Haidaris
Mar 10, 2006·The Journal of Biological Chemistry·Hai Ou Duan, Patricia J Simpson-Haidaris
Oct 12, 2007·American Journal of Physiology. Cell Physiology·Jane SottileJennifer Chandler
Apr 29, 1998·The Journal of Biological Chemistry·A SahniC W Francis
Aug 25, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Laurel E AverettOleg V Gorkun

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