Abstract
Plasmin sensitive sites are found on the A alpha, B beta and gamma chains of fibrinogen at regions joining the two C-terminal D fragments with the central E fragment. We have developed a monoclonal antibody (MoAb) reactive with this plasmin sensitive region on the human fibrinogen gamma chain and mapped its epitope. MoAb J88B reacts with gamma chains of both native as well as with reduced and denatured fibrinogen and fibrin, the CNBr fragment of the fibrinogen central domain, plasmin cleaved fragments D, gamma-gamma dimers, but not with plasmic fragments E. These data indicate that J88B maps to the plasmin sensitive domain localized to gamma 63-78. MoAb J88B failed to react with synthetic peptide gamma 70-78, which suggests that the epitope includes the newly exposed N-terminal residues gamma 63-70 of the early plasmic fragment D1A. As calcium has a marked influence on plasmin cleavage of C-terminal sites on the gamma chain, the effects of calcium on modulating plasmin cleavage of D1A to D1 were assessed in the absence or presence of J88B. The results indicated that calcium delays and J88B (+/- calcium) protects the gamma chain from plasmin cleavage at the N-terminus of D1A, suggesting that this enzymatically labile site is cal...Continue Reading
References
Sep 29, 1979·Biochimica Et Biophysica Acta·C S Cierniewski, T S Edgington
Jun 1, 1977·Thrombosis Research·F Haverkate, G Timan
Jan 25, 1979·Biochimica Et Biophysica Acta·S A OlexaV J Marder
Jan 1, 1976·Annual Review of Biochemistry·M Morrison, G R Schonbaum
Sep 1, 1992·Thrombosis Research·N YoshidaM Okuma
Jul 2, 1985·Biochemistry·J W Donovan, E Mihalyi
Feb 11, 1986·Biochemistry·A Váradi, H A Scheraga
Feb 9, 1988·Biochemistry·E Mihalyi
Feb 9, 1988·Biochemistry·E Mihalyi
Jul 2, 1985·Biochemistry·E Mihalyi, J W Donovan
Jul 1, 1971·The Journal of Clinical Investigation·M L SchwartzP A McKee
Jan 1, 1984·Molecular Immunology·B KudrykM E Wiebe
Nov 1, 1980·The Journal of Clinical Investigation·C W FrancisG H Barlow
Feb 27, 1981·Biochimica Et Biophysica Acta·W NieuwenhuizenF Haverkate
Jun 27, 1983·Annals of the New York Academy of Sciences·W Nieuwenhuizen, F Haverkate
Jul 1, 1983·Thrombosis Research·W NieuwenhuizenJ Hermans
Jul 14, 1995·Cell·Z RaoD Stuart
Sep 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Rémy S PasquetPaul Gepts
Citations
Jan 12, 2001·Thrombosis Research·A E MichelsenF Brosstad
Oct 27, 2009·Archives of Biochemistry and Biophysics·Barbara CardinaliMattia Rocco
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·A Sahni, C W Francis
Oct 24, 2007·Journal of Thrombosis and Haemostasis : JTH·A SahniC W Francis
Dec 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·T M OdrljinP J Simpson-Haidaris
Aug 6, 2003·The Journal of Biological Chemistry·Hai Ou Duan, Patricia J Simpson-Haidaris
Mar 10, 2006·The Journal of Biological Chemistry·Hai Ou Duan, Patricia J Simpson-Haidaris
Oct 12, 2007·American Journal of Physiology. Cell Physiology·Jane SottileJennifer Chandler
Feb 7, 2004·Blood·Nobuo OkumuraSusan T Lord
Mar 25, 2004·Blood·Abha SahniCharles W Francis
Apr 29, 1998·The Journal of Biological Chemistry·A SahniC W Francis
Aug 25, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Laurel E AverettOleg V Gorkun