Calcium oscillation linked to pacemaking of interstitial cells of Cajal: requirement of calcium influx and localization of TRP4 in caveolae

The Journal of Biological Chemistry
S TorihashiShinsuke Nakayama

Abstract

Interstitial cells of Cajal (ICC) are considered to be pacemaker cells in gastrointestinal tracts. ICC generate electrical rhythmicity (dihydropyridine-insensitive) as slow waves and drive spontaneous contraction of smooth muscles. Although cytosolic Ca(2+) has been assumed to play a key role in pacemaking, Ca(2+) movements in ICC have not yet been examined in detail. In the present study, using cultured cell clusters isolated from mouse small intestine, we demonstrated Ca(2+) oscillations in ICC. Fluo-4 was loaded to the cell cluster, the relative amount of cytosolic Ca(2+) was recorded, and ICC were identified by c-Kit immunoreactivity. We specifically detected Ca(2+) oscillation in ICC in the presence of dihydropyridine, which abolishes Ca(2+) oscillation in smooth muscles. The oscillation was coupled to the electrical activity corresponding to slow waves, and it depended on Ca(2+) influx through a non-selective cation channel, which was SK&F 96365-sensitive and store-operated. We further demonstrated the presence of transient receptor potential-like channel 4 (TRP4) in caveolae of ICC. Taken together, the results infer that the Ca(2+) oscillation in ICC is intimately linked to the pacemaker function and depends on Ca(2+) in...Continue Reading

References

Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·L BirnbaumerM Birnbaumer
Dec 1, 1995·Pflügers Archiv : European journal of physiology·N TokutomiK Nishi
Dec 31, 1997·The Journal of Physiology·S NakayamaT Tomita
Jul 14, 1998·Nature Medicine·L ThomsenJ D Huizinga
Apr 1, 1999·Annual Review of Physiology·B HorowitzK M Sanders
Mar 16, 2000·Trends in Neurosciences·C HarteneckG Schultz
May 19, 2000·The Journal of Biological Chemistry·S PhilippV Flockerzi
Jul 27, 2000·American Journal of Physiology. Cell Physiology·A EppersonB Horowitz
Oct 4, 2000·The Japanese Journal of Physiology·H Suzuki
Apr 5, 2001·American Journal of Physiology. Cell Physiology·R L WalkerB Horowitz
May 2, 2001·Nature Reviews. Genetics·R M Bush
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Oct 16, 2001·American Journal of Physiology. Cell Physiology·A FujitaF Hata

❮ Previous
Next ❯

Citations

May 9, 2007·Pflügers Archiv : European journal of physiology·Indu S Ambudkar, Hwei Ling Ong
May 18, 2010·Cell Biochemistry and Biophysics·Xu HuangZong-Lai Jiang
Mar 2, 2010·Archives of Pharmacal Research·Sun Young ParkUy Dong Sohn
Jan 3, 2006·Cardiovascular Research·Christopher D Hardin, Johana Vallejo
Apr 28, 2004·Cell Calcium·T B BoltonV Pucovsky
Jun 20, 2007·Antioxidants & Redox Signaling·Michael J MorganZhenggang Liu
Jun 16, 2011·Cardiovascular Research·Jessica SabourinEric Raddatz
Oct 23, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joel Abramowitz, Lutz Birnbaumer
Dec 15, 2006·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Seok ChoiJae Yeoul Jun
Jul 17, 2004·Biological & Pharmaceutical Bulletin·Hirofumi MorinoTomoo Kuge
Jun 5, 2010·Biological & Pharmaceutical Bulletin·Byung Joo KimSeon Jeong Kim
Mar 3, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Kei-ichiro NakamuraYosaburo Shibata
Mar 3, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Shinsuke NakayamaYuji Imaizumi
Mar 3, 2004·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Toshiko Yamazawa, Masamitsu Iino
Sep 29, 2011·PloS One·Hong-Nian LiuShinsuke Nakayama
Jun 3, 2010·Frontiers in Bioscience (Landmark Edition)·Jose C Gonzalez-Cobos, Mohamed Trebak
Aug 4, 2010·Journal of Neurogastroenterology and Motility·Seok ChoiJae Yeoul Jun
May 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Akikazu FujitaToyoshi Fujimoto
Jan 16, 2013·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Othman A Al-Shboul
Mar 29, 2014·Naunyn-Schmiedeberg's Archives of Pharmacology·Pawan Kumar ShahiJae Yeoul Jun
Aug 11, 2006·Canadian Journal of Physiology and Pharmacology·Geoffrey BoddyE E Daniel
Dec 26, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Miyako Takaki
May 17, 2006·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Byung Joo KimKi Whan Kim
Nov 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Vladimir RiazanskiDeborah J Nelson
Mar 25, 2004·Journal of Cellular Physiology·Maria-Giuliana VannucchiMaria-Simonetta Faussone-Pellegrini
Dec 7, 2014·Science China. Life Sciences·Jie FuMichael X Zhu
Aug 14, 2010·Journal of Theoretical Biology·Shawn A Means, James Sneyd
May 15, 2010·Neurochemistry International·Tamera K Bardell, Eric L Barker
Jun 18, 2010·Progress in Biophysics and Molecular Biology·Miyako TakakiShinsuke Nakayama
Jul 7, 2009·Biosensors & Bioelectronics·Shinsuke NakayamaKenzo Hirose
Feb 24, 2009·European Journal of Pharmacology·Dan-Thanh T NguyenBetty Exintaris
Mar 21, 2007·Cell Calcium·Sylvie Cayouette, Guylain Boulay
Dec 19, 2006·Biochemical and Biophysical Research Communications·Aya HottaHikaru Suzuki

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.