PMID: 8939352Oct 1, 1996Paper

Calcium oscillations in melanotrope cells of Xenopus laevis are differentially regulated by cAMP-dependent and cAMP-independent mechanisms

Cell Calcium
J R LiesteE W Roubos

Abstract

Intracellular Ca2+ oscillations play an important role in the induction of alpha-MSH release from pituitary melanotrope cells of Xenopus laevis. Oscillatory, secretory and adenylyl cyclase activities are all inhibited by dopamine, neuropeptide Y (NPY) and baclofen (a GABAB receptor agonist) and stimulated by sauvagine. In this study, we test the hypothesis that these neural messengers regulate the Ca2+ oscillations via a cAMP/protein kinase A (PKA)-dependent mechanism. To this end, video-imaging microscopy was applied to single Xenopus melanotropes loaded with the Ca2+ indicator Fura-2. The cAMP-dependent PKA inhibitor H89 blocked Ca2+ oscillations as well as the stimulatory actions of 8-Br-cAMP and sauvagine. Treatment of cells inhibited by baclofen with either 8-Br-cAMP or sauvagine led to a reappearance of Ca2+ oscillations. A similar result was found for cells inhibited by NPY. Neither 8-Br-cAMP nor sauvagine induced Ca2+ oscillations in cells inhibited by dopamine. Depolarizing dopamine-inhibited cells with high potassium also failed to induce oscillations, but combining 8-Br-cAMP with membrane depolarization induced oscillations. It is concluded that sauvagine, baclofen and NPY work primarily through a cAMP/PKA-pathway wh...Continue Reading

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Citations

Apr 26, 2003·General and Comparative Endocrinology·Bruce G JenksWim J J M Scheenen
Dec 24, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·E W Roubos
May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S M KolkE W Roubos
Oct 10, 2002·Journal of Neuroendocrinology·L N CornelisseW J J M Scheenen
Jun 18, 1998·Annals of the New York Academy of Sciences·B JenksE Roubos
Jun 18, 1998·Annals of the New York Academy of Sciences·H J LeendersE W Roubos
Mar 29, 2007·Neuroendocrinology·Bruce G JenksEric W Roubos
May 9, 2012·Journal of Neuroendocrinology·S S StojilkovicC A Stratakis

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