Calcium phosphate embedded PLGA nanoparticles: a promising gene delivery vector with high gene loading and transfection efficiency

International Journal of Pharmaceutics
Jie TangYu Zheng

Abstract

In the purpose of increasing incorporation efficiency and improving the release kinetics of plasmid DNA (pDNA) from poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles, a facile method for the fabrication of calcium phosphate (CaPi) embedded PLGA nanoparticles (CaPi-pDNA-PLGA-NPs) was developed. The effect of several preparation factors on the particle size, incorporation efficiency, pDNA release and transfection efficiency in vitro was studied by Single Factor Screening Method. These preparation factors included the molecular weight (MW), hydrolysis degree (HD) of polyvinyl alcohol (PVA), sonication power and time, composition of organic phase, initial concentration of calcium phosphate and calcium (Ca) to phosphate ion (P) ratio (Ca/P ratio), etc. The CaPi-pDNA-PLGA-NPs made according to the optimal formulation were spherical in shape observed by transmission electron microscopy (TEM) with a mean particle size of 207±5 nm and an entrapment efficiency of 95.7±0.8%. Differential scanning calorimetry (DSC) suggested that there existed interaction between the DNA-calcium-phosphate (CaPi-pDNA) complexes and the polymeric matrices of PLGA. X-ray diffractometry (XRD) further proved the conclusion and indicated that the CaPi-pDNA was...Continue Reading

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Citations

May 24, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jie TangZhi Ping Xu
Feb 18, 2020·Expert Opinion on Drug Delivery·David Medina-CruzThomas J Webster
Nov 18, 2018·Pharmaceutical Research·Larissa Gomes Dos ReisDaniela Traini
Dec 21, 2017·Chemical Society Reviews·Chao QiPeng Huang
Nov 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Gregor DoerdelmannMatthias Epple
Jun 30, 2015·Advanced Healthcare Materials·Sue Hyun LeeJae Won Yang
May 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wenxiu HouXiangyang Shi
Feb 13, 2021·Chemistry : a European Journal·Viktoriya Sokolova, Matthias Epple
Dec 16, 2020·Pharmaceutical Nanotechnology·Sweet NaskarKetousetuo Kuotsu

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