Calcium phosphate scaffolds with defined interconnecting channel structure provide a mimetic 3D niche for bone marrow metastasized tumor cell growth

Acta Biomaterialia
Sanja AveicHorst Fischer

Abstract

Metastasis of tumor cells in the bone marrow (BM) is a multi-step and highly dynamic process during which cells succumb important phenotypic changes. Behavior of disseminated tumor cells in BM is strictly regulated by three-dimensional (3D) cell-cell and cell-matrix interactions. In this study, we explored whether the β-tricalcium-phosphate (β-TCP) scaffolds with a tailored interconnecting channel structure could enable appropriate 3D mimetic BM microenvironment for the growth of metastatic neuroblastoma cells. The scaffolds provided the mechanical support for human mesenchymal stromal cells (hMSC) allowing them to proliferate, differentiate towards osteoblasts, and produce the deposits of extracellular matrix inside the interconnected channels. The in vitro microenvironment shaped by stromal cells was then tailored by neuroblastoma tumor cells. Immunohistological analyses confirmed the organization of tumor cells into the forms of spheres only when co-cultured with hMSC-derived osteoblasts. The growing rate of tumor cells in 3D conditions was less marked comparing to the one of the cells grown as 2D monolayer as confirmed by decreased Ki-67 expression. Instead, the 3D culturing of neuroblastoma cells inside supportive stroma p...Continue Reading

Citations

Sep 25, 2020·International Journal of Molecular Sciences·Ushashi C DadwalJulie A Rhoades
Sep 15, 2020·Journal of Extracellular Vesicles·Marta CollettiAngela Di Giannatale
Dec 17, 2020·Frontiers in Immunology·Diana CoralloSanja Aveic
Oct 29, 2020·Journal of Experimental & Clinical Cancer Research : CR·Amparo López-CarrascoRosa Noguera
Mar 16, 2021·Current Opinion in Biomedical Engineering·Kalpana S KattiDinesh R Katti

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