Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate.

The Journal of Biological Chemistry
Dragomir MilovanovicGeert van den Bogaart

Abstract

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a minor component of total plasma membrane lipids, but it has a substantial role in the regulation of many cellular functions, including exo- and endocytosis. Recently, it was shown that PI(4,5)P2and syntaxin 1, a SNARE protein that catalyzes regulated exocytosis, form domains in the plasma membrane that constitute recognition sites for vesicle docking. Also, calcium was shown to promote syntaxin 1 clustering in the plasma membrane, but the molecular mechanism was unknown. Here, using a combination of superresolution stimulated emission depletion microscopy, FRET, and atomic force microscopy, we show that Ca(2+)acts as a charge bridge that specifically and reversibly connects multiple syntaxin 1/PI(4,5)P2complexes into larger mesoscale domains. This transient reorganization of the plasma membrane by physiological Ca(2+)concentrations is likely to be important for Ca(2+)-regulated secretion.

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Citations

Oct 22, 2016·Journal of Molecular Biology·Alf Honigmann, Arnd Pralle
Oct 27, 2016·Nature Reviews. Immunology·Wei WuChenqi Xu
Feb 9, 2017·Journal of the American Chemical Society·Eva BilkovaÜnal Coskun
Mar 16, 2018·Traffic·Muhmmad Omar-HmeadiSebastian Barg
Nov 25, 2017·Frontiers in Immunology·Yuanqing MaKatharina Gaus
May 5, 2017·Disease Models & Mechanisms·Sofia V ZaichickGabriela Caraveo
Feb 7, 2017·Frontiers in Physiology·Jing HanRainer A Böckmann
Aug 28, 2020·Frontiers in Synaptic Neuroscience·Georgii NosovJurgen Klingauf
Apr 29, 2021·Chemical Communications : Chem Comm·Yusuke ShimizuKazushi Kinbara
May 23, 2021·Science Advances·Alessandro MoroMatthijs Verhage
Jan 15, 2021·Annual Review of Biochemistry·Yi WenGerald W Feigenson
Sep 30, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Maria J SarmentoFábio Fernandes

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Methods Mentioned

BETA
atomic force microscopy
FRET
AFM

Software Mentioned

Fiji

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