Calcium pyrophosphate dihydrate crystals activate MAP kinase in human neutrophils: inhibition of MAP kinase, oxidase activation and degranulation responses of neutrophils by taxol

Immunology
J K JacksonH M Burt

Abstract

The activation of MAP kinase in human neutrophils stimulated by both uncoated and plasma-opsonized crystals of triclinic calcium pyrophosphate dihydrate (CPPD) was investigated. The effect of taxol on MAP kinase activation and on the responses of neutrophils stimulated by plasma-opsonized crystals was determined. MAP kinase activation was identified and quantified in Mono Q chromatography separated fractions of neutrophils that had been incubated with CPPD crystals by measuring [gamma-32P]adenosine triphosphate (ATP) phosphorylation of myelin basic protein and using immunoblotting techniques. Human neutrophils were incubated with taxol (0-50 microM), added to plasma-opsonized CPPD (50 mg/ml) and MAP kinase activation, chemiluminescence, superoxide anion generation, lysozyme and myeloperoxidase release were monitored. Both uncoated and plasma coated CPPD crystals induced a large increase in MAP kinase activity in neutrophils over control levels within 1 min of incubation. Pretreatment of neutrophils with taxol was able to suppress this activation of MAP kinase. Taxol produced a concentration-dependent inhibition of opsonized CPPD-induced neutrophil chemiluminescence, superoxide anion production and myeloperoxide release. Taxol a...Continue Reading

References

Feb 22, 1979·Nature·P B SchiffS B Horwitz
Aug 1, 1991·Journal of Cellular Physiology·D A JurivichJ Blenis
Nov 15, 1989·Biochemical and Biophysical Research Communications·W D Blackburn, L W Heck
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
Sep 1, 1982·The Journal of Cell Biology·J J ManfrediS B Horwitz
Sep 1, 1995·Trends in Biochemical Sciences·S W Edwards
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·A A ReszkaE H Fischer
Nov 27, 1995·International Journal of Cancer. Journal International Du Cancer·K NishioN Saijo
May 1, 1995·Current Opinion in Rheumatology·J M Houri, F X O'Sullivan
Jan 1, 1994·Critical Reviews in Biochemistry and Molecular Biology·S Ferrari, G Thomas
Jun 1, 1994·Arthritis and Rheumatism·E BrahnM L Banquerigo
Aug 1, 1994·The Journal of Clinical Investigation·G S WorthenG L Johnson
Feb 2, 1996·The Journal of Biological Chemistry·C KnallG L Johnson
Nov 1, 1995·Trends in Biochemical Sciences·J L Bos
Dec 1, 1995·Current Opinion in Cell Biology·M M Chou, J Blenis
Jan 1, 1996·Rheumatology International·C I WinternitzH M Burt
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M P ScheidV Duronio

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Citations

Jul 2, 2005·Joint, Bone, Spine : Revue Du Rhumatisme·Ru Liu-Bryan, Frédéric Lioté
Feb 27, 1999·Current Opinion in Cell Biology·G G Gundersen, T A Cook
Jan 16, 2004·The New England Journal of Medicine·Gregg W StoneUNKNOWN TAXUS-IV Investigators
May 1, 2002·Current Opinion in Rheumatology·Maria P Morgan, Geraldine M McCarthy
Apr 20, 2005·Current Opinion in Rheumatology·Nicola Dalbeth, Dorian O Haskard
Dec 13, 2002·Journal of Interventional Cardiology·Eberhard Grube, Lutz Büllesfeld
Apr 26, 2006·The American Journal of Cardiology·Achille GaspardoneOlga Skossyreva

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