PMID: 6408220May 1, 1983Paper

Calcium reduces the sodium permeability of luminal membrane vesicles from toad bladder. Studies using a fast-reaction apparatus

The Journal of General Physiology
H S Chase, Q Al-Awqati

Abstract

Regulation of the sodium permeability of the luminal membrane is the major mechanism by which the net rate of sodium transport across tight epithelia is varied. Previous evidence has suggested that the permeability of the luminal membrane might be regulated by changes in intracellular sodium or calcium activities. To test this directly, we isolated a fraction of the plasma membrane from the toad urinary bladder, which contains a fast, amiloride-sensitive sodium flux with characteristics similar to those of the native luminal membrane. Using a flow-quench apparatus to measure the initial rate of sodium efflux from these vesicles in the millisecond time range, we have demonstrated that the isotope exchange permeability of these vesicles is very sensitive to calcium. Calcium reduces the sodium permeability, and the half-maximal inhibitory concentration is 0.5 microM, well within the range of calcium activity found in cells. Also, the permeability of the luminal membrane vesicles is little affected by the ambient sodium concentration. These results, when taken together with studies on whole tissue, suggest that cell calcium may be an important regulator of transepithelial sodium transport by its effect on luminal sodium permeabilit...Continue Reading

References

Jul 26, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S Grinstein, D Erlij
Jul 1, 1979·The Journal of General Physiology·S I HelmanR S Fisher
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·R W Meech
Mar 10, 1978·The Journal of Membrane Biology·K TurnheimS G Schultz
Sep 25, 1978·The Journal of Membrane Biology·S M Thompson, D C Dawson
Sep 1, 1977·The Journal of General Physiology·K R Spring, G Giebisch
May 7, 1976·Analytical Biochemistry·O D GaskoE Racker
Aug 27, 1976·The Journal of Membrane Biology·S A LewisJ M Diamond
Dec 1, 1971·The Journal of General Physiology·S G McLaughlinG Eisenman
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R LatorreC Hidalgo
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S Miyazaki, Y Igusa
Mar 8, 1982·Biochimica Et Biophysica Acta·E F Labelle, S O Lee
Jan 1, 1982·Journal of Cellular Biochemistry·V Bennett
Sep 2, 1980·Biochimica Et Biophysica Acta·E F LaBelle, M E Valentine
Jan 1, 1980·The Journal of Membrane Biology·B Lindemann
Jun 2, 1958·Acta Physiologica Scandinavica·V KOEFOED-JOHNSEN, H H USSING
Nov 1, 1961·Acta Physiologica Scandinavica·E A MACROBBIE, H H USSING

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Citations

May 1, 1990·Pflügers Archiv : European journal of physiology·T ShimizuM Imai
Jan 1, 1985·Pflügers Archiv : European journal of physiology·H F Schoen, D Erlij
Mar 1, 1985·Pflügers Archiv : European journal of physiology·D R Halm, D C Dawson
Feb 1, 1985·Pflügers Archiv : European journal of physiology·S M Sanioto, J Aboulafia
Sep 15, 1986·Klinische Wochenschrift·E WindhagerC O Lee
Jan 1, 1985·The Journal of Membrane Biology·E KelepourisM M Civan
Jan 1, 1986·The Journal of Membrane Biology·H Garty
Apr 1, 1991·The Journal of Membrane Biology·M M CivanT G O'Brien
Jul 1, 1989·The Journal of Membrane Biology·G A Morduchowicz, N Yanagawa
Jan 1, 1987·The Journal of Membrane Biology·H Garty, M M Civan
Jan 1, 1984·The Journal of Membrane Biology·H Garty
Jan 1, 1990·Comparative Biochemistry and Physiology. A, Comparative Physiology·H F Bjerregaard, R Nielsen
Jan 1, 1994·Ultrasound in Medicine & Biology·A M al-KarmiJ C Matthews
Dec 13, 2003·Cell Calcium·U Subrahmanyeswara RaoPremalatha Balachandran
Aug 1, 1989·Baillière's Clinical Endocrinology and Metabolism·K D Mitchell, L G Navar
Jan 1, 1991·Annals of the New York Academy of Sciences·E E WindhagerS Milovanovic
Apr 1, 1985·The Journal of Clinical Investigation·J E ScobleK A Hruska
Oct 1, 1995·Kidney International·B J Harvey
Sep 22, 2017·The Journal of General Physiology·Lawrence G Palmer
Aug 15, 2001·American Journal of Physiology. Renal Physiology·N L NakhoulL L Hamm
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Nov 18, 2000·American Journal of Physiology. Cell Physiology·M S AwaydaL L Hamm
Jan 1, 1990·Journal of Internal Medicine. Supplement·H WienerC H van Os
Apr 1, 1988·The American Journal of Physiology·C AsherH Garty
Jun 11, 1986·The American Journal of Physiology·S M Wong, H S Chase
Jun 1, 1987·The American Journal of Physiology·W Nagel
Jun 1, 1988·The American Journal of Physiology·D S LesterH Garty
Mar 1, 1989·The American Journal of Physiology·M M CivanT G O'Brien
Feb 1, 1986·The American Journal of Physiology·S Sariban-Sohraby, D J Benos
Jul 20, 2021·Journal of Cellular Physiology·Jun ZhangZhi-Ren Zhang

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