PMID: 8969900Nov 1, 1996Paper

Calcium regulates human CYP11B2 transcription

Endocrine Research
C ClyneWilliam E Rainey

Abstract

The CYP11B2 gene encodes aldosterone synthase, a cytochrome P450 (P450aldo) expressed in high levels in the adrenal zona glomerulosa. While the primary physiologic regulators of aldosterone production are circulating angiotensin II (Ang II) and potassium (K+) the action of these agents on CYP11B2 gene transcription have not been examined. Because these factors increase intracellular calcium we have hypothesized that calcium signaling pathways are one mechanism controlling CYP11B2 transcription. Previously we demonstrated that increases in intracellular calcium increase P450aldo mRNA. Herein, we analyzed the role of calcium in the expression of the human CYP11B2 gene using transient transfection of a luciferase reporter construct containing 2017 bp of human CYP11B2 5'flanking DNA in mouse Y-1 and human H295R adrenocortical cell lines. When transfected into Y-1 cells, reporter gene expression was increased following treatment with ACTH or forskolin, but not with Ang II, the L-type calcium channel agonist BAYK8644, or ionomycin. In H295R cells, however, reporter gene expression was increased following treatment with Ang II, K+, BAYK8644 ionomycin or dibutyryl cAMP (Bu2cAMP). Activation of protein kinase C with TPA did not alter re...Continue Reading

References

Feb 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T KawamotoH Imura
May 31, 1990·Biochemical and Biophysical Research Communications·N MatsukawaM Okamoto
Jun 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J G LeHouxA Lefebvre
Apr 1, 1994·Molecular and Cellular Endocrinology·W E RaineyJ I Mason
Feb 1, 1994·Molecular and Cellular Endocrinology·K G MountjoyR D Cone
Aug 1, 1993·Molecular and Cellular Endocrinology·O B HollandW E Rainey
Apr 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·G Aguilera

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Citations

Oct 3, 2000·Endocrinology·X L WangW E Rainey
Mar 27, 2004·Physiological Reviews·András Spät, László Hunyady
Mar 28, 1998·The Journal of Biological Chemistry·J S SilvestreC Delcayre
Dec 3, 2003·Molecular Endocrinology·Mary H BassettWilliam E Rainey
Oct 6, 2006·Journal of the American Society of Nephrology : JASN·Josée MichaudVincent Pichette
Mar 6, 2010·Journal of Biomedicine & Biotechnology·Takehiro KoKenichiro Kitamura
Jan 27, 2004·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H M KorashyA O S El-Kadi
Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Francois A LeblondVincent Pichette
Jul 20, 1999·Molecular and Cellular Endocrinology·W E Rainey
Feb 7, 2001·Journal of the American Society of Nephrology : JASN·Francois LeblondVincent Pichette
Mar 18, 2004·Molecular and Cellular Endocrinology·András Spät, János G Pitter

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