Calcium regulates the mycophagous ability of Burkholderia gladioli strain NGJ1 in a type III secretion system-dependent manner.

BMC Microbiology
Sunil Kumar YadavGopaljee Jha

Abstract

A rice associated bacterium Burkholderia gladioli strain NGJ1 demonstrates mycophagy, a phenomenon wherein bacteria feed on fungi. Previously, we have reported that NGJ1 utilizes type III secretion system (T3SS) to deliver a prophage tail-like protein (Bg_9562) into fungal cells to establish mycophagy. In this study, we report that calcium ion concentration influences the mycophagous ability of NGJ1 on Rhizoctonia solani, an important fungal pathogen. The calcium limiting condition promotes mycophagy while high calcium environment prevents it. The expression of various T3SS apparatus encoding genes of NGJ1 was induced and secretion of several potential T3SS effector proteins (including Bg_9562) into extracellular milieu was triggered under calcium limiting condition. Using LC-MS/MS proteome analysis, we identified several calcium regulated T3SS effector proteins of NGJ1. The expression of genes encoding some of these effector proteins was upregulated during mycophagous interaction of NGJ1 with R. solani. Further, mutation of one of these genes (endo-β-1, 3- glucanase) rendered the mutant NGJ1 bacterium defective in mycophagy while complementation with full length copy of the gene restored its mycophagous activity. Our study pro...Continue Reading

References

Sep 1, 1990·Infection and Immunity·T MichielsG Cornelis
Jan 1, 1984·The American Journal of Physiology·D J Miller, G L Smith
Jan 15, 1980·Biochemical and Biophysical Research Communications·R BarrF L Crane
Jun 1, 1993·Molecular Microbiology·S C StraleyK A Fields
Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·C J Hueck
Jul 27, 2001·Applied and Environmental Microbiology·W De BoerJ A Van Veen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 8, 2004·Molecular Microbiology·Delfina C Dominguez
Mar 11, 2005·Nature Reviews. Microbiology·Dieter Haas, Geneviève Défago
Jun 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mark L UrbanowskiTimothy L Yahr
Nov 8, 2005·Microbiology·Jaewha KimShouguang Jin
Sep 26, 2006·Molecular Microbiology·Timothy L Yahr, Matthew C Wolfgang
Dec 1, 2006·Nature·Jorge E Galán, Hans Wolf-Watz
Dec 19, 2007·The New Phytologist·Johan H J Leveau, Gail M Preston
Oct 10, 2008·Bioinformatics·Geoffrey L WinsorFiona S L Brinkman
Oct 24, 2008·Phytopathology·P P SinghY R Chung
Apr 25, 2009·PLoS Pathogens·Roland ArnoldThomas Rattei
Jun 2, 2009·Journal of Molecular Biology·Riffat NaseemAnthony K Campbell
Jun 16, 2009·PloS One·Martin Löwer, Gisbert Schneider
Aug 15, 2009·Nature Reviews. Microbiology·Alan R Hauser
Aug 18, 2009·Applied and Environmental Microbiology·Silvia VilchesSusana Merino
Aug 20, 2010·The ISME Journal·Gerald LacknerChristian Hertweck
Sep 21, 2010·Journal of Bacteriology·Cindy J Gode-PotratzLinda L McCarter
May 4, 2011·Current Opinion in Plant Biology·Barbara Reinhold-Hurek, Thomas Hurek
Oct 13, 2011·Molecular Systems Biology·Fabian SieversDesmond G Higgins
Dec 1, 2011·Microbiology and Molecular Biology Reviews : MMBR·P Frey-KlettA Sarniguet
Aug 2, 2012·Antimicrobial Agents and Chemotherapy·Miles C DuncanVictoria Auerbuch
Feb 12, 2014·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Guo-Feng JiangJi-Liang Tang
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pei-Chung LeeArne Rietsch
Jul 9, 2014·Annual Review of Microbiology·Jorge E GalánSamuel Wagner
Jan 4, 2015·Cell Calcium·Delfina C DomínguezMarianna Patrauchan
Aug 19, 2015·Frontiers in Microbiology·Soumitra Paul ChowdhuryRainer Borriss
Nov 29, 2015·Nucleic Acids Research·UNKNOWN NCBI Resource Coordinators

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Methods Mentioned

BETA
PCR

Software Mentioned

Mascot
msconvert
Clustal Omega
EffectiveT3
BioNJ
PRIMER
MEGAX

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