Calcium regulation in the intraerythrocytic malaria parasite Plasmodium falciparum

Molecular and Biochemical Parasitology
L M Alleva, Kiaran Kirk

Abstract

The regulation of intracellular Ca(2+) in the intraerythrocytic form of the human malaria parasite, Plasmodium falciparum, was investigated using parasites 'isolated' from their host cells by saponin-permeabilisation of the erythrocyte membrane. The isolated parasites maintained tight control over their resting cytosolic Ca(2+) concentration which ranged from approximately 100 nM in the absence of extracellular Ca(2+) to approximately 700 nM in the presence of 1 mM extracellular Ca(2+). The parasite has two functionally discrete intracellular Ca(2+) stores. One is an 'endoplasmic reticulum (ER)-like' store, the other an 'acidic store'. The ER-like store was discharged by cyclopiazonic acid (CPA), an inhibitor of sarco/endoplasmic reticulum Ca(2+)-ATPases (SERCAs) of animal and plant cells, but not by thapsigargin (TG), a more specific inhibitor of SERCAs of animal cells. The acidic store was discharged by nigericin and by NH(4)(+). The amount of Ca(2+) in the ER-like store increased with increasing extracellular Ca(2+) concentration, whereas the amount of Ca(2+) in the acidic store did not. Ca(2+) released from the ER-like store by CPA was cleared from the parasite cytosol by uptake into the acidic store (over a range of extrac...Continue Reading

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Citations

May 4, 2004·Blood Cells, Molecules & Diseases·Virgilio L LewTeresa Tiffert
Aug 4, 2004·Molecular and Biochemical Parasitology·Laurence FlorensYimin Wu
Apr 7, 2005·Molecular and Biochemical Parasitology·Shirley L FariasCélia R S Garcia
Sep 22, 2001·International Journal for Parasitology·S KrishnaC Woodrow
Sep 22, 2001·International Journal for Parasitology·K J Saliba, K Kirk
Dec 31, 2003·Lancet·Odile Mercereau-Puijalon, Thierry Fandeur
Jan 18, 2003·Cell Calcium·Fernando P VarottiCélia R S Garcia
Aug 28, 2003·Current Opinion in Microbiology·Silvia N J Moreno, Roberto Docampo
Nov 5, 2004·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Felix A RuizRoberto Docampo
Nov 26, 2003·Antimicrobial Agents and Chemotherapy·Christopher P LocherPhilip J Rosenthal
Dec 23, 2015·Cellular Signalling·Alexandre BuduMarcos L Gazarini
Aug 5, 2008·Molecular and Biochemical Parasitology·Natalie J SpillmanKiaran Kirk
Sep 1, 2007·Experimental Parasitology·Kildare MirandaSilvia N J Moreno
Mar 12, 2008·Traffic·Lilach Sheiner, Dominique Soldati-Favre
May 22, 2010·Molecular Microbiology·Alexander RotmannMichael Lanzer
Nov 4, 2004·Trends in Parasitology·Katja Becker, Kiaran Kirk
Sep 11, 2004·Biochemical and Biophysical Research Communications·Marcos L Gazarini, Célia R S Garcia
Jun 29, 2004·Biochemical and Biophysical Research Communications·Adele M LehaneKiaran Kirk
Jun 10, 2009·Biotechnology Journal·Petra Rohrbach
May 31, 2014·BioMed Research International·Sabna CheemadanZbynek Bozdech
Jan 22, 2015·Cell Calcium·Sebastian Lourido, Silvia N J Moreno
May 16, 2012·Microbes and Infection·Alexandre Budu, Célia R S Garcia
Dec 12, 2013·The Biochemical Journal·Kiaran Kirk, Adele M Lehane
May 16, 2012·Microbes and Infection·Anthony A HolderJudith L Green
Oct 11, 2011·Molecular and Biochemical Parasitology·Piero BagnaresiCélia R S Garcia
Oct 22, 2015·Annual Review of Microbiology·Kiaran Kirk
Apr 16, 2003·The Journal of Cell Biology·Patricia Camacho
Apr 16, 2003·The Journal of Cell Biology·Marcos L GazariniCélia R S Garcia
Mar 24, 2016·Scientific Reports·Kishor PandeyKazuhide Yahata
Jul 11, 2013·International Journal of Molecular Sciences·Wânia Rezende LimaCélia R S Garcia
Nov 25, 2003·The Journal of Biological Chemistry·Richard J W Allen, Kiaran Kirk
Mar 21, 2018·Antimicrobial Agents and Chemotherapy·Adelaide S M DennisKiaran Kirk

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