PMID: 2507513Sep 1, 1989Paper

Calcium regulation of skeletal myogenesis. I. Cell content critical to myotube formation

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
R J PrzybylskiA C Kirby

Abstract

Primary cultures of embryonic chick pectoral skeletal muscle were used to study calcium regulation of myoblast fusion to form multinucleated myotubes. Using atomic absorption spectrometry to measure total cellular calcium and the 45Ca-exchange method to determine free cellular Ca++, our data suggest that only the free cellular calcium changes significantly during development under conditions permissive for myotube formation (0.9 mM external Ca++). Increases in calcium uptake occurred before and toward the end of the period of fusion with the amount approximating 2 to 4 pmol per cell in mass cultures. If the medium [Ca++] is decreased to 0.04 mM, as determined with a calcium electrode, a fusion-block is produced and free cell Ca++ decreased 5- to 10-fold. Removal of the fusion-block by increasing medium [Ca++] results in a release of the fusion-block and an increase in cellular Ca++ to approximately 1 pmol per cell during fusion, and higher thereafter. Cation ionophore A23187 produced transient increases in cellular calcium and stimulated myoblast fusion and the final extent of myotube formation only when added at the onset of culture. Results suggest that transient increased calcium uptake alone is insufficient for fusion becau...Continue Reading

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Citations

Jul 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R G MacBride
Jun 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·P BijlengaL Bernheim
Mar 13, 2002·The Journal of Cell Biology·Hiromichi WadaShigetake Sasayama
May 3, 2000·The Journal of Cell Biology·B B FridayG K Pavlath
Feb 17, 2015·Biotechnology Progress·Christopher W McAleerJames J Hickman
Jul 16, 2013·Muscle & Nerve·Bao-Ting ZhangElla W Yeung
Mar 1, 1992·Experimental Cell Research·S W ChoiM S Kang
Jul 4, 2003·American Journal of Physiology. Cell Physiology·Werner J H KoopmanPeter H G M Willems
Dec 6, 2001·The Journal of Biological Chemistry·Ad J C de GroofWerner J H Koopman
Feb 14, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Keiko NakanishiNobuhiro Morishima
Oct 15, 2011·Muscle & Nerve·Kwok-Kuen CheungElla W Yeung
Nov 13, 2004·Journal of Neuropathology and Experimental Neurology·Noemí De LunaIsabel Illa
Feb 28, 2019·BMC Genomics·Sara de Las Heras-SaldanaCedric Gondro

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