Calcium regulation of tension redevelopment kinetics with 2-deoxy-ATP or low [ATP] in rabbit skeletal muscle

Biophysical Journal
M RegnierP B Chase

Abstract

The correlation of acto-myosin ATPase rate with tension redevelopment kinetics (k(tr)) was determined during Ca(+2)-activated contractions of demembranated rabbit psoas muscle fibers; the ATPase rate was either increased or decreased relative to control by substitution of ATP (5.0 mM) with 2-deoxy-ATP (dATP) (5.0 mM) or by lowering [ATP] to 0.5 mM, respectively. The activation dependence of k(tr) and unloaded shortening velocity (Vu) was measured with each substrate. With 5.0 mM ATP, Vu depended linearly on tension (P), whereas k(tr) exhibited a nonlinear dependence on P, being relatively independent of P at submaximum levels and rising steeply at P > 0.6-0.7 of maximum tension (Po). With dATP, Vu was 25% greater than control at Po and was elevated at all P > 0.15Po, whereas Po was unchanged. Furthermore, the Ca(+2) sensitivity of both k(tr) and P increased, such that the dependence of k(tr) on P was not significantly different from control, despite an elevation of Vu and maximal k(tr). In contrast, lowering [ATP] caused a slight (8%) elevation of Po, no change in the Ca(+2) sensitivity of P, and a decrease in Vu at all P. Moreover, k(tr) was decreased relative to control at P > 0.75Po, but was elevated at P < 0.75Po. These dat...Continue Reading

References

Jan 1, 1992·Pharmacology & Therapeutics·J M Chalovich
Aug 1, 1992·Journal of Muscle Research and Cell Motility·Z GrabarekJ Gergely
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H L Sweeney, J T Stull
Mar 1, 1985·The Journal of Physiology·I MatsubaraN Yagi
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B Brenner, E Eisenberg
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B Brenner
Mar 1, 1988·The Journal of General Physiology·F V BrozovichA M Gordon
Jun 1, 1988·Biophysical Journal·P B Chase, M J Kushmerick
Jun 1, 1988·Journal of Muscle Research and Cell Motility·A J FarrowJ Unsworth
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R F SiemankowskiH D White
Oct 1, 1971·The American Journal of Physiology·R M WiseF N Briggs
Jul 1, 1984·The Journal of Physiology·M A FerencziD R Trentham
Jun 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S Farah, F C Reinach
Jan 1, 1996·Annual Review of Physiology·L S Tobacman
Jan 16, 1998·Journal of Muscle Research and Cell Motility·W O HancockA M Gordon

❮ Previous
Next ❯

Citations

Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sarah G NowakowskiMichael Regnier
Feb 26, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Julien OchalaLars Larsson
Jul 17, 2007·PLoS Computational Biology·Bertrand C W TannerMichael Regnier
Dec 15, 2015·Journal of Molecular and Cellular Cardiology·Daria Amiad Pavlov, Amir Landesberg
May 2, 2014·Journal of Molecular and Cellular Cardiology·Scott D LundyMichael Regnier
Sep 20, 2011·Journal of Molecular and Cellular Cardiology·F S KorteMichael Regnier
Oct 18, 2011·Journal of Molecular and Cellular Cardiology·Anthony J Baker
Nov 3, 2009·Upsala Journal of Medical Sciences·Julien OchalaLars Larsson
Mar 4, 2006·Biophysical Journal·Julian E StelzerRichard L Moss
Nov 3, 2010·Journal of Molecular and Cellular Cardiology·Kareen L KreutzigerMichael Regnier
Jan 18, 2017·Protein Science : a Publication of the Protein Society·Sarah G NowakowskiValerie Daggett
Jan 6, 2007·The Journal of Physiology·Alicia Moreno-GonzalezMichael Regnier
May 23, 2002·FEBS Letters·Anna E BukatinaGary C Sieck
Jun 24, 2000·Circulation Research·M RegnierP B Chase
Jun 7, 2003·Circulation Research·Ozgur Ogut, Frank V Brozovich
Oct 30, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Rashid NassarPage A W Anderson
Sep 24, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Anthony G RodriguezMichael Regnier
Feb 16, 2005·Journal of Muscle Research and Cell Motility·Emilie Warner Clemmens, Michael Regnier
Nov 21, 2008·Rapid Communications in Mass Spectrometry : RCM·Andrzej Pelc, Stanislaw Halas
Jan 13, 2010·Journal of Physics. Condensed Matter : an Institute of Physics Journal·J RohrerP Hyldgaard
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Jochen Rohrer, Per Hyldgaard
May 22, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joseph D PowersMichael Regnier

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.