Calcium regulation of the ATPase activity of Physarum and scallop myosins using hybrid smooth muscle myosin: the role of the essential light chain

FEBS Letters
Ying ZhangKazuhiro Kohama

Abstract

To examine the role of two light chains (LCs) of the myosin II on Ca2+ regulation, we produced hybrid heavy meromyosin (HMM) having LCs from Physarum and/or scallop myosin using the smooth muscle myosin heavy chain. Ca2+ inhibited motility and ATPase activity of hybrid HMMs with LCs from Physarum myosin but activated those of hybrid HMM with LCs from scallop myosin, indicating an active role of LCs. ATPase activity of hybrid HMMs with LCs from different species showed the same effect by Ca2+ even though they did not support motility. Our results suggest that communication between the original combinations of LC is important for the motor function.

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Citations

Nov 15, 2011·Journal of Molecular Biology·Bruce A J BaumannKenneth A Taylor
Dec 13, 2016·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Kazuhiro Kohama
Oct 23, 2018·Nano Letters·Eric R KuhnBhanu P Jena
Nov 2, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ying ZhangSei Kobayashi

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