PMID: 8996408Feb 1, 1997Paper

Calcium regulation of urinary bladder function

The Journal of Urology
M S DamaserR M Levin

Abstract

To investigate the effect of independently inhibiting calcium influx from extracellular sources and calcium release from intracellular stores on the ability of the urinary bladder to generate pressure and empty. Rabbit bladders were mounted in an in vitro whole organ bath and filled with 15 ml. saline. Each bladder was incubated separately in Tyrode's solution, with diltiazem (10 microM), to block extracellular calcium influx, or with thapsigargin (40 microM) and ryanodine (80 microM), to block the uptake and release of calcium from the sarcoplasmic reticulum. The bladder was then stimulated isometrically with field stimulation (32 Hz), and to empty with field stimulation and with bethanechol (250 microM), independently. During stimulation, transmural pressure and volume emptied were measured. From these, flow rate, power, and external mechanical work were calculated. In the presence of diltiazem, the time to maximal pressure decreased while the rate of pressure generation increased. This results from increased participation of intracellular calcium release, which occurs rapidly and near the smooth muscle filaments, decreasing the diffusion time. In the presence of thapsigargin and ryanodine the maximal rate of pressure generat...Continue Reading

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Citations

Sep 10, 2013·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·James T KearnsMohan S Gundeti
Jun 18, 2002·European Journal of Pharmacology·Sefa ResimAtilla Dikmen
Aug 5, 1999·British Journal of Pharmacology·J G MastersJ I Gillespie
Dec 16, 2000·Expert Opinion on Investigational Drugs·A J Wein
Sep 1, 1997·The Journal of Urology·H J YuM S Damaser
Jul 23, 2004·Physiological Reviews·Karl-Erik Andersson, Anders Arner

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