PMID: 6974735Sep 1, 1981Paper

Calcium release and ionic changes in the sarcoplasmic reticulum of tetanized muscle: an electron-probe study

The Journal of Cell Biology
A V SomlyoA P Somlyo

Abstract

Approximately 60-70% of the total fiber calcium was localized in the terminal cisternae (TC) in resting frog muscle as determined by electron-probe analysis of ultrathin cryosections. During a 1.2 s tetanus, 59% (69 mmol/kg dry TC) of the calcium content of the TC was released, enough to raise total cytoplasmic calcium concentration by approximately 1 mM. This is equivalent to the concentration of binding sites on the calcium-binding proteins (troponin and parvalbumin) in frog muscle. Calcium release was associated with a significant uptake of magnesium and potassium into the TC, but the amount of calcium released exceeded the total measured cation accumulation by 62 mEq/kg dry weight. It is suggested that most of the charge deficit is apparent, and charge compensation is achieved by movement of protons into the sarcoplasmic reticulum (SR) and/or by the movement of organic co- or counterions not measured by energy dispersive electron-probe analysis. There was no significant change in the sodium or chlorine content of the TC during tetanus. The unchanged distribution of a permeant anion, chloride, argues against the existence of a large and sustained transSR potential during tetanus, if the chloride permeability of the in situ S...Continue Reading

References

Aug 22, 1977·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R MilediG Schalow
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·H Gonzalez-SerratosA P Somlyo
Apr 1, 1978·The Journal of General Physiology·E W Stephenson
Jul 4, 1979·Biochimica Et Biophysica Acta·J M GillisC Gosselin-Rey
Apr 21, 1975·Biochimica Et Biophysica Acta·G Meissner
Jan 1, 1975·Annual Review of Biophysics and Bioengineering·D H MacLennan, P C Holland
Feb 1, 1979·The Journal of Cell Biology·A O JorgensenD H MacLennan
Apr 1, 1979·European Journal of Biochemistry·T BeelerA Martonosi
Jul 1, 1979·The Journal of General Physiology·B R Eisenberg, A Gilai
Jan 31, 1978·Pflügers Archiv : European journal of physiology·A DörgeK Thurau
Jan 1, 1978·Annual Review of Biophysics and Bioengineering·R H Adrian
Dec 15, 1978·The Journal of Membrane Biology·D McKinley, G Meissner
Jan 1, 1975·Progress in Biophysics and Molecular Biology·L L Costantin
Jun 1, 1969·Biochemistry·R HerzI Reiss
Aug 1, 1970·The Journal of General Physiology·P F Duggan, A Martonosi
Jan 1, 1968·The Journal of General Physiology·S Winegrad
Jan 1, 1968·Annual Review of Physiology·L D Peachey
Jan 1, 1968·Progress in Biophysics and Molecular Biology·S Ebashi, M Endo
Jan 2, 1970·Science·L E Ford, R J Podolsky
Jan 1, 1970·The Journal of General Physiology·S Winegrad
Mar 1, 1970·The Journal of Cell Biology·R J PodolskyS L Hatchett
May 1, 1968·The Journal of General Physiology·F Fuchs, F N Briggs
Jun 1, 1965·The Journal of Cell Biology·L D Peachey
May 1, 1980·Journal of Ultrastructure Research·J R SommerJ Junker
Jan 1, 1980·The Journal of Membrane Biology·K P Campbell, A E Shamoo
Jul 1, 1980·The Journal of General Physiology·R T MathiasR S Eisenberg
May 1, 1980·Journal of Cellular Physiology·M R James-KrackeA P Somlyo
Sep 30, 1980·The Journal of Membrane Biology·V C ChiuD H Haynes
Oct 15, 1980·Journal of Molecular Biology·G HamoirB Focant
Mar 25, 1957·The Journal of Biophysical and Biochemical Cytology·K R PORTER, G E PALADE

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Citations

Jan 1, 1987·European Journal of Applied Physiology and Occupational Physiology·S E AlwayJ S Frank
Jan 1, 1983·Histochemistry·E Horowitz, M B Engel
Dec 1, 1982·Pflügers Archiv : European journal of physiology·E E Bittar, J Nwoga
Aug 1, 1987·Pflügers Archiv : European journal of physiology·R H Fink, D G Stephenson
Jan 1, 1984·Pflügers Archiv : European journal of physiology·J Y Su, W Hasselbach
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·P Volpe
Aug 31, 1994·Molecular and Cellular Biochemistry·C GongR M Levin
Jan 1, 1982·The Journal of Membrane Biology·H Miyamoto, E Racker
Jul 15, 1985·Experientia·A V Somlyo, C Franzini-Armstrong
May 15, 1985·Experientia·K S Cheah, A M Cheah
Jan 1, 1983·Annals of Biomedical Engineering·A P SomlyoD Kowarski
Jan 14, 2010·Pflügers Archiv : European journal of physiology·Ramón Jiménez-MorenoOsvaldo Delbono
Jan 18, 2012·Pflügers Archiv : European journal of physiology·Zhong-Min WangOsvaldo Delbono
Dec 18, 2012·Pflügers Archiv : European journal of physiology·Elisa VenturiHiroshi Takeshima
Nov 1, 1990·Developmental Biology·J E SpeksnijderL F Jaffe
Jan 1, 1985·Progress in Biophysics and Molecular Biology·J CailléO Rougier
Jan 1, 1986·Progress in Biophysics and Molecular Biology·F Ramón, A Rivera
Feb 1, 1996·Acta Astronautica·T YoshiokaK Yamashita-Goto
Oct 1, 1988·Trends in Neurosciences·M P Blaustein
Oct 1, 1988·Trends in Neurosciences·M Fill, R Coronado
Jan 1, 1982·Ultramicroscopy·A P Somlyo, H Shuman
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S WatabeK Hashimoto
Jun 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·M Charbonneau
Jan 1, 1997·Progress in Biophysics and Molecular Biology·J I Kourie
Jan 1, 1989·Progress in Histochemistry and Cytochemistry·B Walz, O Baumann
Jan 25, 2003·Cell Calcium·C CamelloA V Tepikin
Feb 1, 1991·Quarterly Reviews of Biophysics·C C AshleyT J Lea
Jun 17, 1998·Nature Structural Biology·D H MacLennan, R A Reithmeier
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H Shuman, A P Somlyo
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A MaurerS Fleischer

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