PMID: 7580872Aug 1, 1995Paper

Calcium responses of isolated, immunocytochemically identified rat pinealocytes to noradrenergic, cholinergic and vasopressinergic stimulations

Neurochemistry International
C SchomerusH W Korf

Abstract

Calcium responses of isolated rat pineal cells to noradrenergic, cholinergic and vasopressinergic stimulations were recorded by use of the fura-2 technique and an image analysis system. Subsequently the recorded cells were identified as pinealocytes by immunocytochemical demonstration of S-antigen, a pinealocyte-specific marker. S-antigen immunoreactive pinealocytes were shown to respond to norepinephrine stimulation with an elevation of the intracellular free calcium concentration ([Ca2+]i). This response was dose-dependent and consisted of a rapid increase in [Ca2+]i (primary phase) followed by a decrease to an elevated plateau well above the basal level (secondary phase). The plateau persisted for at least 1 h when cells were constantly exposed to norepinephrine and dropped to basal level upon removal of the stimulus. Analysis of the calcium responses of cells treated with caffeine or thapsigargin suggested that the primary phase reflects mobilization of calcium from inositol 1,4,5-trisphosphate-sensitive intracellular calcium stores. Depletion of these calcium stores was a decisive and sufficient prerequisite to evoke the secondary phase which was apparently elicited by calcium influx. These data suggest that a capacitative...Continue Reading

References

Oct 1, 1992·Trends in Biochemical Sciences·C W Taylor, I C Marshall
Jan 1, 1992·Progress in Brain Research·H W Korf, H Wicht
May 1, 1992·Microscopy Research and Technique·M Møller
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
Feb 28, 1989·European Journal of Pharmacology·J T LaitinenJ M Saavedra
Jan 1, 1989·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·P GovitrapongM Ebadi
Nov 1, 1989·The Journal of Endocrinology·L M FinocchiaroC J Pirola
Jun 28, 1974·Science·J Axelrod
Jun 15, 1973·Brain Research·H J Romijn
Jun 23, 1980·Life Sciences·R L TaylorD R Burt
Mar 1, 1994·Journal of Pineal Research·J C SáezD C Spray
Jun 1, 1993·Current Opinion in Neurobiology·R PennerM Hoth

❮ Previous
Next ❯

Citations

Mar 1, 1997·Journal of Neuroscience Research·P RedeckerS Steinlechner
Apr 24, 2009·Cell and Tissue Research·S SchönigerF Nürnberger
May 14, 1997·Regulatory Peptides·H R RahmaniC D Ingram
Mar 21, 1997·Neuroscience Letters·I WesslerL Vollrath
Oct 21, 1998·Neuroscience Letters·J Schenda, L Vollrath
May 1, 1995·Journal of Pineal Research·J H Stehle
Jun 17, 2008·Journal of Pineal Research·Marco KochHorst-Werner Korf
Jul 11, 2013·International Journal of Molecular Sciences·Wânia Rezende LimaCélia R S Garcia
Oct 4, 2000·Physical Review Letters·S Mertens
Sep 7, 2005·Cell Biochemistry and Function·Solange C AfecheJosé Cipolla-Neto
Nov 12, 2005·American Journal of Physiology. Cell Physiology·So-Young LeeKyong-Tai Kim
Feb 21, 2014·American Journal of Physiology. Cell Physiology·Jin-Young YoonDuk-Su Koh
Sep 1, 1999·Microscopy Research and Technique·P Phansuwan-PujitoP Govitrapong
Mar 10, 2001·Microscopy Research and Technique·F Nürnberger, S Schöniger
Dec 1, 1996·Die Naturwissenschaften·H W KorfJ H Stehle

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.