Calcium rise in human platelets elicited by anti-CD9 and -CD41 murine monoclonal antibodies

Thrombosis Research
R FavierM Samama

Abstract

Three murine monoclonal antibodies (anti-CD9: ALB6, anti-CD41: VI-PL3 and PL2-49/GPIIb - final concentration: 7.5 micrograms/mL) are shown to elicit after a lag time aggregation of washed platelets and a calcium signal (as detected by light emitted by loaded aequorin), which is only partially inhibited by aspirin. By comparison the rise induced by thrombin is greater and almost instantaneous. In the presence of EGTA a calcium mobilization from internal stores can be detected with thrombin and with ALB6, but neither with PL2-49 nor with VI-PL3, whereas platelets still change their shape and release ATP. It is tempting to speculate that although all the antibodies induce a calcium change, they activate platelets by different pathways: calcium may be not primarily involved in the activation induced by the anti-CD41 antibodies.

References

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Citations

May 24, 1991·Biochimica Et Biophysica Acta·L Y Chau, J R Lin
Mar 15, 2005·Platelets·Gregory W Moseley
Aug 9, 2020·Journal of Thrombosis and Haemostasis : JTH·Pravin PatelUlhas P Naik
Aug 31, 1990·Biochemical and Biophysical Research Communications·Y YatomiK Kurokawa

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