Calcium-sensing receptor antagonist NPS-2143 suppresses proliferation and invasion of gastric cancer cells.

Cancer Gene Therapy
Zong-Lin ZhangSu-Rong Wang

Abstract

NPS-2143 is a calcium-sensing receptor (CaSR) antagonist that has been demonstrated to possess anticancer activity. To date, the effects of NPS-2143 on gastric cancer (GC) cell growth, motility, and apoptosis have not been investigated. In the present study, we firstly investigated the expression of CaSR in GC tissues using immunohistochemistry and western blotting. Then Cell Counting Kit-8 and colony formation assays were conducted to explore the effect of the NPS-2143 on the proliferation of GC cell line AGS. Transwell invasion and migration assays were performed to test the effect of NPS-2143 on AGS cell motility. We determined the percentage of apoptotic cells by flow cytometry and explored the changes of apoptosis-related protein by western blotting. Furthermore, we constructed a CaSR knockdown AGS cell line to determine whether NPS-2143 acted via inhibition of CaSR. We found that the protein expression level of CaSR was higher in GC tissues compared with the paired adjacent normal tissues. In addition, NPS-2143 treatment caused an inhibitory effect on the proliferation, invasion, and migration of AGS cells and a promoting effect on AGS apoptosis. The expression of Bcl-2 was decreased while the levels of Bax and active cas...Continue Reading

References

Mar 1, 1995·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·E M BrownS C Hebert
Feb 17, 2007·International Journal of Cancer. Journal International Du Cancer·Ming-Tsan LinMin-Liang Kuo
Jun 9, 2007·International Journal of Cancer. Journal International Du Cancer·Narasimharao BhagavathulaJames Varani
Nov 18, 2008·Endocrine·Bandana ChakravartiNaibedya Chattopadhyay
Jun 2, 2011·British Journal of Pharmacology·Donald T Ward, Daniela Riccardi
Dec 27, 2012·Biochimica Et Biophysica Acta·Sarah C BrennanEnikö Kallay
Dec 18, 2013·Pharmacology & Therapeutics·Jiri Polivka, Filip Janku
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Apr 24, 2015·Science Translational Medicine·Polina L YarovaDaniela Riccardi
Jul 15, 2015·Scandinavian Journal of Gastroenterology·Chung Hyun TaeSung-Ae Jung
Aug 9, 2015·Critical Reviews in Oncology/hematology·Lei ChangYong Li
Nov 27, 2015·Biochimica Et Biophysica Acta·Samawansha TennakoonEnikö Kállay
May 10, 2016·Lancet·Eric Van CutsemHans Prenen
Dec 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jinfeng GanHao Zhang
Feb 28, 2019·Journal of Pharmacological Sciences·Aya YamamuraMotohiko Sato

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Citations

Oct 28, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·R XieJ Xu
Mar 3, 2020·Frontiers in Oncology·Souvik DasRomuald Mentaverri
Oct 30, 2020·Cells·Jesús Cosín-RogerSara Calatayud

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Datasets Mentioned

BETA
GES-1

Methods Mentioned

BETA
flow cytometry
Protein Assay
electrophoresis
transfection

Software Mentioned

SPSS

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