Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells

Bone
Hiroyuki MizumachiHidefumi Maeda

Abstract

Activation of the G protein-coupled calcium-sensing receptor (CaSR) has crucial roles in skeletal development and bone turnover. Our recent study has identified a role for activated CaSR in the osteogenic differentiation of human periodontal ligament stem cells. Furthermore, odontoblasts residing inside the tooth pulp chamber play a central role in dentin formation. However, it remains unclear how CaSR activation affects the odontoblastic differentiation of human dental pulp cells (HDPCs). We have investigated the odontoblastic differentiation of HDPCs exposed to elevated levels of extracellular calcium (Ca) and strontium (Sr), and the contribution of CaSR and the L-type voltage-dependent calcium channel (L-VDCC) to this process. Immunochemical staining of rat dental pulp tissue demonstrated that CaSR was expressed at high levels in the odontoblastic layer, moderate levels in the sublayer, and low levels in the central pulp tissue. Although normal HDPCs expressed low levels of CaSR, stimulation with Ca or Sr promoted both CaSR expression and odontoblastic differentiation of HDPCs along with increased expression of odontoblastic makers. These effects were inhibited by treatment with a CaSR antagonist, whereas treatment with an L...Continue Reading

Citations

Aug 6, 2018·Journal of Cellular Physiology·Aoi NozuHidefumi Maeda
Mar 14, 2019·Expert Reviews in Molecular Medicine·Angeliki Vogiatzi, George Mavrothalassitis
Jul 19, 2019·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Qun DingShuanrang Jing
Oct 16, 2019·Journal of Cellular Physiology·Shoko FujinoHidefumi Maeda
Mar 4, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Cing-Yu ChenYuk-Man Leung
May 23, 2019·Cell Proliferation·Yi-Zhou TanBei Li
Feb 1, 2021·Biochemical and Biophysical Research Communications·So Mi JeonJong-Ho Lee
Apr 29, 2021·Journal of Dental Research·F S HosseiniC T Laurencin
May 4, 2021·The Chinese Journal of Physiology·Iat-Lon LeongYuk-Man Leung

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