Calcium sensing receptor mediated the excessive generation of β-amyloid peptide induced by hypoxia in vivo and in vitro

Biochemical and Biophysical Research Communications
Shuai BaiYougen Luo

Abstract

Hypoxia played an important role in the pathogenesis of AD. Hypoxia increased Aβ formation, then caused Alzheimer's disease. Calcium sensing receptor (CaSR) was involved in the regulation of cell growth, differentiation, hormonal secretion and other physiological function. Increasing evidence supported CaSR might play a more prominent role in susceptibility to AD, but the role of CaSR in Aβ overproduction induced by hypoxia and its mechanisms remain unclear. To investigate whether CaSR mediated the overproduction of Aβ induced by hypoxia, immunoblot and immunochemistry were employed to determine the expression of CaSR and BACE1 in hippocampal neurons and tissue and Ca(2+) image system was used to measure [Ca(2+)]i in hippocampal neurons. The content of Aβ was detected with ELISA kits. Our research found that hypoxia increased the expression of CaSR in hippocampal neurons and tissue and [Ca(2+)]i in hippocampal neurons. Calhex 231, a selective blocher of CaSR, inhibited the increase in [Ca(2+)]i induced by hypoxia. Hypoxia or GdCl3, an agonist of CaSR, increased the expression of BACE1 in hippocampal neurons and tissue, but Calhex 231 or Xesto C (a selective inhibitor of IP3 receptor) partly prevented hypoxia-induced BACE1 overe...Continue Reading

References

Apr 15, 1993·Nature
Jun 18, 2004·Cell Calcium·Shozo YanoNaibedya Chattopadhyay
Nov 17, 2007·Neuroreport·Yougen LuoJianzhi Wang
Nov 28, 2008·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Yvette P ConleyRobert E Ferrell
Mar 9, 2010·Trends in Molecular Medicine·David P Stirling, Peter K Stys
Apr 21, 2010·Brain : a Journal of Neurology·Jessica M Mc DonaldUNKNOWN Medical Research Council Cognitive Function and Ageing Study
Nov 6, 2013·Cell Calcium·Michael Henrich, Keith J Buckler
Dec 30, 2014·Annals of Clinical and Translational Neurology·Jong Youl KimWenhan Chang
Dec 31, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Shiyun DongWeihua Zhang

❮ Previous
Next ❯

Citations

Apr 12, 2016·Frontiers in Physiology·Brian L Jones, Stephen M Smith
May 21, 2016·Frontiers in Physiology·Anna ChiariniIlaria Dal Prà
Jan 27, 2017·Orphanet Journal of Rare Diseases·C VaheM C Vantyghem
Aug 12, 2016·Physiological Reviews·Michael J Berridge
Jul 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Lo GiudiceJulianna Kobolák
Oct 24, 2019·Journal of Alzheimer's Disease : JAD·Maria Lo GiudiceJulianna Kobolák
Mar 4, 2019·Naunyn-Schmiedeberg's Archives of Pharmacology·Cing-Yu ChenYuk-Man Leung
Dec 3, 2020·International Journal of Molecular Sciences·Anna ChiariniIlaria Dal Prà

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Alzheimer's Disease: Amyloid Beta

Alzheimer's disease is a neurodegenerative disease associated with the accumulation of amyloid plaques in the brain; these plaques are comprised of amyloid beta deposits. Here is the latest research in this field.