Calcium-sensing receptor stimulates luminal K+-dependent H+ excretion in medullary thick ascending limbs of Henle's loop of mouse kidney

The Tohoku Journal of Experimental Medicine
Elnur Ilham FarajovY Kondo

Abstract

The calcium-sensing receptor (CaSR) is known well as a sensor of extracellular calcium for regulating parathyroid hormone secretion. CaSR is located along all nephron segments in the kidney. While hypercalcemia strongly enhances urinary acidification, the relationship between CaSR and acid-base metabolism in the kidney is still uncertain. In the present study, we examined whether CaSR activation caused acid secretion in the medullary thick ascending limb (mTAL), which is one of the major nephron segments involved in both mineral and acid-base regulation. The effects of a potent calcimimetic neomycin (Neo) on intracellular pH (pHi) were analyzed in the in vitro miroperfused mouse mTALs. The mTALs were incubated with 2,7-bis-(2-carboxyethyl)-5(6)-carboxyfluoresceine-acetoxymethylester (BCECF-AM) for microfluorescent pHi measurements. In HCO(3)(-)/CO(2)-buffered solution, the steady-state pHi was 7.17 +/- 0.01 (n = 19). Basolateral Neo at 0.4 mM in basolateral side significantly alkalinized the mTAL cells to 7.28 +/- 0.02 (n = 19), while Neo in the lumen had no effect on pHi. Neo in the basolateral side alkalinized the mTALs in the absence of ambient Na(+) and the presence of H(+)-ATPase inhibitor bafilomycin in the lumen, indicat...Continue Reading

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Citations

Jun 5, 2012·Current Opinion in Nephrology and Hypertension·Giuseppe VezzoliLaura Soldati
Nov 20, 2009·American Journal of Physiology. Renal Physiology·Daniela Riccardi, Edward M Brown
May 28, 2009·Journal of the American Society of Nephrology : JASN·Kirsten Y RenkemaJoost G Hoenderop
Mar 7, 2014·Canadian Journal of Physiology and Pharmacology·Fernando CasareRicardo Fernandez
Dec 15, 2015·American Journal of Physiology. Renal Physiology·J A Z GracaS A Price
Jul 2, 2018·Clinical and Experimental Nephrology·Priscilla Marys Costa Dos SantosRicardo Fernandez

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