PMID: 2500640Mar 1, 1989Paper

Calcium sensitivity and energetics of contraction in skinned smooth muscle of the guinea pig taenia coli at altered pH

Pflügers Archiv : European journal of physiology
H ArhedenP Hellstrand

Abstract

Calcium-sensitivity of contraction, force-velocity relation and ATP hydrolysis rate at different pH (6.2-7.8) were investigated in skinned smooth muscle preparations from the guinea pig taenia coli. Varied free-calcium levels were buffered by 4 mM BAPTA (1,2-bis(2-aminophenoxy)-ethane-N,N,N'N'-tetraacetic acid) which has calcium binding properties little affected by pH. A small increase of calcium-sensitivity of contraction was seen at pH 6.2 compared to 6.9 and 7.8 (ED50 shift of about 0.15 pCa units). The isometric force and Vmax in fibres activated either by calcium or by thiophosphorylation of the myosin light chains were each reduced by about 15% at pH 6.2 compared to 6.9 and 7.8. Following an isotonic quick release the shortening velocity decreases with time. This effect was more pronounced at pH 6.2 than at pH 6.9 or 7.8. The ATP hydrolysis rates in relaxed and thiophosphorylated fibres were essentially unaffected by alteration in pH between 6.2 and 7.8. Due to the lower force, energetic cost of force maintenance was thus somewhat increased at pH 6.2. These results suggest that pH alteration between 6.2 and 7.8 have effects on the properties of the contractile machinery of the smooth muscle in the skinned guinea pig taen...Continue Reading

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Citations

Oct 1, 1992·Pflügers Archiv : European journal of physiology·R C HeatonS Wray
May 1, 1993·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·H Nguyen Duong
Jul 23, 2004·Physiological Reviews·Karl-Erik Andersson, Anders Arner
Feb 16, 1999·Journal of Neurotrauma·R L MacdonaldB Weir
Jan 1, 1993·The American Journal of Physiology·S Wray
Jan 1, 1995·The American Journal of Physiology·S Morimoto, Y Ogawa

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