Calcium signal induced by mechanical perturbation of osteoclasts

Journal of Cellular Physiology
S L Xia, J Ferrier

Abstract

Multinucleated osteoclasts from rabbit long bone, 1-6 days in culture, respond to mechanical perturbation with a transient increase of intracellular calcium concentration ([Ca2+]i), as measured with the fluorescent indicator fluo-3 on a confocal laser scanning microscope. In experiments with different extracellular calcium concentrations (from 11.8 mM to calcium-free), the incidence, the magnitude, and the duration of [Ca2+]i responses decreases with decreasing bathing [Ca2+]. Following mechanical perturbation, a thapsigargin-induced [Ca2+]i response has a lower magnitude than the thapsigargin-induced response without mechanical perturbation. In thapsigargin-pretreated osteoclasts the mechanical perturbation-induced rise in [Ca2+]i is larger and longer than in control cells. Ni2+ inhibits the incidence and decreases both the magnitude and the duration of the responses, while nifedipine, verapamil, and Gd3+ have no effect. These measurements show that rabbit osteoclasts transduce a mechanical perturbation of the cell membrane into a [Ca2+]i signal via both a calcium influx and an internal calcium release.

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Citations

Oct 3, 1999·Medical & Biological Engineering & Computing·A J el HajS J Publicover
Apr 26, 2012·Annals of Biomedical Engineering·Ping LiDing Zhang
Dec 28, 1999·Journal of Biomechanics·T D Brown
Apr 9, 2014·Annals of Biomedical Engineering·Ping LiBo Huo
Dec 10, 1999·Experimental Cell Research·W RaddingH C Blair
Nov 16, 2010·Biochimica Et Biophysica Acta·Sung-Yong Hwang, James W Putney
Nov 7, 2016·European Journal of Pharmacology·Futoshi IwakiKiyoshi Ohura
Apr 1, 1996·Journal of Cellular Physiology·S L Xia, J Ferrier
Oct 29, 2021·Journal of Biomedical Nanotechnology·Qingxuan WangChenchen Zhou

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