Calcium signalling in wound-responsive normal human urothelial cell monolayers

Cell Calcium
Saqib Shabir, Jennifer Southgate

Abstract

Epithelial tissue repair requires coordination of migratory and proliferative activity both adjacent to and remote from the wound edge. Although calcium signalling is implicated, the specific mechanisms are poorly understood. This study characterises the calcium signal invoked in response to scratch wounding of normal human urothelial (NHU) cells and relates it to the localised cellular response. Immediately after wounding of confluent NHU cell monolayers, cells adjacent to the wound edge showed a sustained (>30 min) rise in [Ca(2+)](i), while there was an independent, but simultaneous calcium wave that propagated out from the wound edge. The transient signal involved release of calcium from intracellular stores and was not mediated via gap junctions, but by diffusion of extracellular agonists. We demonstrated that ATP was partially responsible for the initiation and propagation of the calcium wave and showed that the calcium release mechanism was mediated in part via activation of inositol-1,4,5-triphosphate (IP(3)) receptors. By contrast, the sustained calcium signal originated from the extracellular milieu and correlated with an increased rate of migration by these cells. The work presented here provides supportive evidence ...Continue Reading

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Citations

Feb 28, 2013·Nature Reviews. Molecular Cell Biology·João V Cordeiro, António Jacinto
Jul 24, 2013·The Journal of Cell Biology·Marco AntunesAntonio Jacinto
Jul 19, 2013·Journal of the Royal Society, Interface·Peter A ApplebyDawn Walker
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