Calcium signalling regulates the functions of the bZIP protein VIP1 in touch responses in Arabidopsis thaliana

Annals of Botany
Daisuke TsugamaTetsuo Takano

Abstract

VIP1 is a bZIP transcription factor in Arabidopsis thaliana. VIP1 and its close homologues transiently accumulate in the nucleus when cells are exposed to hypo-osmotic and/or mechanical stress. Touch-induced root bending is enhanced in transgenic plants overexpressing a repression domain-fused form of VIP1 (VIP1-SRDXox), suggesting that VIP1, possibly with its close homologues, suppresses touch-induced root bending. The aim of this study was to identify regulators of these functions of VIP1 in mechanical stress responses. Co-immunoprecipitation analysis using VIP1-GFP fusion protein expressed in Arabidopsis plants identified calmodulins as VIP1-GFP interactors. In vitro crosslink analysis was performed using a hexahistidine-tagged calmodulin and glutathione S-transferase-fused forms of VIP1 and its close homologues. Plants expressing GFP-fused forms of VIP1 and its close homologues (bZIP59 and bZIP29) were submerged in hypotonic solutions containing divalent cation chelators, EDTA and EGTA, and a potential calmodulin inhibitor, chlorpromazine, to examine their effects on the nuclear-cytoplasmic shuttling of those proteins. VIP1-SRDXox plants were grown on medium containing 40 mm CaCl2, 40 mm MgCl2 or 80 mm NaCl. MCA1 and MCA2 a...Continue Reading

References

Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·S PetruccelliR N Beachy
Mar 22, 2002·Trends in Plant Science·Marc JakobyUNKNOWN bZIP Research Group
Jul 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tzvi TzfiraVitaly Citovsky
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shunhong DaiRoger N Beachy
Feb 22, 2005·The New Phytologist·A GrabovF Vicente-Agullo
Apr 13, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jianxiong LiVitaly Citovsky
Jan 13, 2006·Current Biology : CB·Elizabeth S Haswell, Elliot M Meyerowitz
Dec 1, 1983·Plant Physiology·J S LeeM L Evans
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Yuko NakagawaHidetoshi Iida
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Shunhong DaiRoger N Beachy
Oct 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andrea PitzschkeHeribert Hirt
Jan 26, 2010·Plant Physiology·Takuya YamanakaHidetoshi Iida
Jun 16, 2010·Journal of Experimental Botany·Yu WuCheng-Bin Xiang
Mar 29, 2012·Plant Physiology·Daisuke TsugamaTetsuo Takano
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Aug 9, 2013·Plant Molecular Biology·Michael James Van OostenAlbino Maggio
Aug 27, 2013·Plant Physiology and Biochemistry : PPB·Daisuke TsugamaTetsuo Takano
Sep 24, 2013·The Protein Journal·Daisuke TsugamaTetsuo Takano
Oct 8, 2013·Current Biology : CB·Carlos S Galvan-AmpudiaChrista Testerink
Jun 24, 2014·The Plant Journal : for Cell and Molecular Biology·Yong ShiStanton B Gelvin
May 21, 2016·Cell·Ronan C O'MalleyJoseph R Ecker
Jul 13, 2016·The Plant Journal : for Cell and Molecular Biology·Paulina Lozano-SotomayorStefan de Folter
Sep 14, 2017·Journal of Experimental Botany·Eun Hyang HanJoshua J Blakeslee

❮ Previous
Next ❯

Citations

Sep 21, 2018·Plant Signaling & Behavior·Daisuke TsugamaTetsuo Takano
Jan 30, 2021·Journal of Experimental Botany·Ritesh GhoshNathalie Leblanc-Fournier
Sep 24, 2018·Biochemical and Biophysical Research Communications·Daisuke TsugamaTetsuo Takano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here