Calcium, Sodium, and Transient Receptor Potential Channel Expression in Human Fetal Midbrain-Derived Neural Progenitor Cells

Stem Cells and Development
Nancy StanslowskyFlorian Wegner

Abstract

Voltage-gated sodium and calcium channels as well as transient receptor potential (TRP) channels are expressed during the differentiation of human neural progenitor cells (hNPCs) and are likely to be involved in regulating neurogenesis. However, the molecular composition of these ion channels in proliferating and differentiating hNPCs is largely unknown. In this study, we investigated fetal mesencephalic hNPCs in respect to their sodium, calcium, and TRP channel subunit expression and function. Quantitative real-time polymerase chain reaction indicated a significant upregulation of voltage-gated sodium and calcium channel subunits in hNPCs after differentiation for 3 weeks in vitro. In contrast, the TRP channel expression did not increase significantly during hNPC maturation. Intracellular Ca2+ measurements showed the marked reduction of KCl-induced Ca2+ transients through inhibition of voltage-gated Ca2+ channels by verapamil and mibefradil in differentiated hNPCs. Application of TRP channel agonists induced intracellular Ca2+ peaks already in proliferating hNPCs without affecting their cell division. The coincubation of hNPCs with TRP channel agonists pregnenolone sulfate or RN1747 did not have any significant effect on their...Continue Reading

References

Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·J H CaldwellS R Levinson
Mar 7, 2001·Nature Cell Biology·H Song, M Poo
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Jul 17, 2001·Current Opinion in Cell Biology·S P YuD W Choi
Dec 10, 2002·Science·Barry J Dickson
Dec 5, 2003·Nature·David E Clapham
Jun 14, 2005·Pflügers Archiv : European journal of physiology·Frank J P KühnAndreas Lückhoff
Jun 18, 2005·The Journal of Physiology·Craig Montell
Mar 24, 2006·Nature·I Scott RamseyDavid E Clapham
Dec 8, 2006·Nature·Nicholas C Spitzer
Sep 10, 2009·Biochemical and Biophysical Research Communications·Fabien VincentMatthew A J Duncton
Aug 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Gunnar HargusOle Isacson
Sep 11, 2012·Brain : a Journal of Neurology·Niels EijkelkampJohn N Wood
Sep 18, 2012·Biochimica Et Biophysica Acta·Roger A Bannister, Kurt G Beam
Feb 13, 2013·The Journal of Biological Chemistry·William A CatterallEvanthia Nanou
Feb 26, 2013·Current Topics in Medicinal Chemistry·Yoshinori TakadaYasuo Mori
Mar 19, 2014·Stem Cell Research & Therapy·Nancy StanslowskyFlorian Wegner
Apr 8, 2015·Pharmacoepidemiology and Drug Safety·Yakun LangYu Fan
Feb 18, 2016·Neural Plasticity·R Ramírez-BarrantesP Olivero
Mar 30, 2016·Cell Calcium·Anna B TothMurali Prakriya
Nov 25, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nancy StanslowskyFlorian Wegner
Jul 18, 2017·Biochemical and Biophysical Research Communications·Sara MirsadeghiSahar Kiani

❮ Previous
Next ❯

Citations

Jul 12, 2018·Stem Cells and Development·Graham C Parker

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay

Software Mentioned

Life Acquisition
Cell F

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.