Calcium-stimulated Cl- secretion in Calu-3 human airway cells requires CFTR

The American Journal of Physiology
S MoonJeffrey J Wine

Abstract

Human airway serous cells secrete antibiotic-rich fluid, but, in cystic fibrosis (CF), Cl(-)-dependent fluid secretion is impaired by defects in CF transmembrane conductance regulator (CFTR) Cl- channels. Typically, CF disrupts adenosine 3',5'-cyclic monophosphate (cAMP)-mediated Cl- secretion but spares Ca(2+)-mediated secretion. However, in CF airway glands, Ca(2+)-mediated secretion is also greatly reduced. To determine the basis of Ca(2+)-mediated Cl- secretion in serous cells, we used thapsigargin to elevate intracellular Ca2+ concentration ([Ca2+]i) in Calu-3 cells, an airway cell line bearing some similarities to serous cells. Cells were cultured using conventional and air interface methods. Short-circuit current (Isc) and transepithelial conductance (Gte) were measured in confluent cell layers. Thapsigargin stimulated large, sustained changes (delta) in Isc and Gte, whereas forskolin stimulated variable and smaller increases. delta Isc was decreased by basolateral bumetanide, quinidine, barium, or diphenylamine-2-carboxylate (DPAC) but was unaffected by high apical concentrations of 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonic acid, and calixarene. Isc was measured after ...Continue Reading

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Citations

Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·N S JooJ J Wine
Dec 8, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert J Lee, J Kevin Foskett
Apr 8, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Amiraj BangaBonnie L Blazer-Yost
Mar 3, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kan SasamoriToshio Hattori
Mar 15, 2006·American Journal of Physiology. Cell Physiology·Melissa L PalmerScott M O'Grady
Mar 10, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·C M LiedtkeT S Cole
Feb 15, 2001·American Journal of Physiology. Cell Physiology·M M Reddy, P M Quinton
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·L TroutS T Ballard
Dec 14, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·B R CobbJ P Clancy
Aug 18, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mauri E KrouseJeffrey J Wine
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Amiraj BangaBonnie L Blazer-Yost
Mar 7, 2014·American Journal of Physiology. Cell Physiology·Christian SchwarzerTerry E Machen
Sep 16, 2011·American Journal of Physiology. Cell Physiology·N Keating, L R Quinlan
May 26, 2007·The Journal of Physiology·Robert J LeeJ Kevin Foskett
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·M Duszyk
Jun 1, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Toshiya IrokawaJeffrey J Wine
Sep 4, 2015·Physiological Reviews·Jonathan H Widdicombe, Jeffrey J Wine
Sep 26, 2000·American Journal of Physiology. Cell Physiology·J LoffingB A Stanton
Feb 6, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sarah K InglisStuart M Wilson
Feb 5, 2002·The Journal of Physiology·Elizabeth A Cowley, Paul Linsdell

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