PMID: 8594540Oct 1, 1995Paper

Calcium transients which accompany the activation of sodium current in rat ventricular myocytes at 37 degrees C: a trigger role for reverse Na-Ca exchange activated by membrane potential?

Pflügers Archiv : European journal of physiology
J C Hancox, A J Levi

Abstract

We investigated the role of the fast sodium current (INa) in triggering Ca release from the sarcoplasmic reticulum (SR), using adult rat left ventricular myocytes, loaded with Fura-2 to measure intracellular Ca (Cai), which were whole-cell patch-clamped at 35-37 degrees C. Before each test pulse, a series of 400-ms conditioning pulses to +10 mV were applied to establish a constant level of SR Ca load. Pulses were applied every 15 s. A test pulse from -80 mV to -50 mV elicited a rapid INa and a phasic Cai transient. When the solution perfusing a myocyte was rapidly switched for 15 s before a test pulse to one containing the L-type Ca channel blocker nifedipine (20 microM), the test pulse still activated INa and a phasic Cai transient, the amplitude of which was not significantly different from control (P > 0.05; t-test). When a rapid switch to 20 microM nifedipine plus 30 microM tetrodotoxin (TTX) was made 15 s before a test pulse, both INa and the Cai transient were completely abolished (n = 6). When a switch was made to Na-free (Li) solution, which contained 20 microM nifedipine to block L-type Ca current, ICa,L, there was no significant difference in the Cai transient amplitude from that of control (P > 0.05; n = 6). Brief de...Continue Reading

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Citations

Sep 1, 1996·Pflügers Archiv : European journal of physiology·A J LeviJ Vinnicombe
Jun 16, 2000·The Journal of Physiology·Y K Ju, D G Allen
Aug 4, 2007·Progress in Neurobiology·Tamás L Török
Mar 15, 2000·Neuroreport·D Wu, P H Zhu
Jul 3, 1999·Physiological Reviews·M P Blaustein, W J Lederer
Aug 13, 1999·The American Journal of Physiology·J A Wasserstrom, A M Vites
Jul 1, 1997·The American Journal of Physiology·E J GriffithsH S Silverman
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·G R Ferrier, S E Howlett

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