Calcium-triggered membrane fusion proceeds independently of specific presynaptic proteins.

The Journal of Biological Chemistry
Joseph A SzuleJens R Coorssen

Abstract

Complexes of specific presynaptic proteins have been hypothesized to drive or catalyze the membrane fusion steps of exocytosis. Here we use a stage-specific preparation to test the roles of SNAREs, synaptotagmin, and SNARE-binding proteins in the mechanism of Ca2+-triggered membrane fusion. Excess exogenous proteins, sufficient to block SNARE interactions, did not inhibit either the Ca2+ sensitivity, extent, or kinetics of fusion. In contrast, despite a limited effect on SNARE and synaptotagmin densities, treatments with high doses of chymotrypsin markedly inhibited fusion. Conversely, low doses of chymotrypsin had no effect on the Ca2+ sensitivity or extent of fusion but did alter the kinetic profile, indicating a more direct involvement of other proteins in the triggered fusion pathway. SNAREs, synaptotagmin, and their immediate binding partners are critical to exocytosis at a stage other than membrane fusion, although they may still influence the triggered steps.

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Citations

Oct 14, 2006·European Biophysics Journal : EBJ·Barry R Lentz
Jul 2, 2009·Journal of Chemical Biology·Kendra L FurberJens R Coorssen
Apr 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Morten Gram Pedersen, Arthur Sherman
Aug 13, 2009·Clinical and Experimental Pharmacology & Physiology·Kendra L FurberJens R Coorssen
Jun 19, 2007·American Journal of Respiratory Cell and Molecular Biology·Pengcheng WangLin Liu
Dec 21, 2005·The Journal of Cell Biology·Christoph Reese, Andreas Mayer
Jan 24, 2009·Annals of the New York Academy of Sciences·Kendra L FurberJens R Coorssen
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Sep 11, 2009·The Biochemical Journal·Matthew A Churchward, Jens R Coorssen
Sep 5, 2014·Molecular Neurobiology·Wei XuJin-Tai Yu
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