PMID: 4266586Apr 1, 1973

Calcium uptake and associated adenosine triphosphatase activity of isolated platelet membranes

The Journal of General Physiology
L S RobbleeF A Belamarich

Abstract

A platelet subcellular fraction, sedimenting between 14,000 and 40,000 g and consisting primarily of membrane vesicles, accumulates up to 200-400 nmoles calcium/mg protein in the presence of ATP and oxalate. Steady-state levels of calcium accumulation are attained in 40-60 min. Calcium uptake requires adenosine triphosphate (ATP), is enhanced by oxalate, and is accompanied by the release of inorganic phosphate. Calcium accumulation and phosphate release require magnesium and are inhibited by Salyrgan (10 microM) and adenosine diphosphate (ADP) (1 mM), but not by ouabain (0.1 mM). The ATPase activity is stimulated by low concentrations of calcium (5-10 microM) and is inhibited by 2 mM EGTA. Electron microscopic histochemistry using lead nitrate to precipitate released phosphate results in lead precipitates localized primarily at the inner surface of membrane vesicles. These results provide evidence for a membrane ATPase that is stimulated by low concentrations of calcium and may be involved in the transport of calcium across the membrane. It is postulated that the observed calcium uptake activity is an in vitro manifestation of a calcium extrusion pump in the intact platelet.

References

Jul 1, 1973·Microvascular Research·L S RobbleeF A Belamarich
Jan 1, 1981·Neuroscience and Biobehavioral Reviews·R D Myers, D H Ross
Jan 1, 1982·Pharmacology & Therapeutics·N G Ardlie
Jan 1, 1990·Comparative Biochemistry and Physiology. A, Comparative Physiology·D W PorterW Kaczmarczyk
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G A Rodan, M B Feinstein
Jul 1, 1977·The Journal of Cell Biology·S E Hitchcock
Jul 1, 1974·The Journal of General Physiology·A M ChambautJ Hanoune
Jan 1, 1976·Clinical and Experimental Pharmacology & Physiology·G Forget, S Heisler
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Mar 1, 1975·Acta Physiologica Scandinavica·J T Russell, N A Thorn
Apr 28, 1978·Annals of the New York Academy of Sciences·L Moore, I Pastan
Jan 1, 1974·CRC Critical Reviews in Biochemistry·T D Pollard, R R Weihing
Oct 1, 1977·Thrombosis Research·L B KornsteinD Shepro
Mar 1, 1979·Prostaglandins and Medicine·A M ButlerJ G White
Nov 1, 1978·Prostaglandins and Medicine·J M GerrardJ G White
Oct 1, 1978·Thrombosis Research·W C Horne, E R Simons
Apr 30, 1984·Biochemical and Biophysical Research Communications·G C White
Nov 15, 1979·FEBS Letters·E Zubrzycka-GaarnH E Osińska
Jan 1, 1977·The American Journal of Physiology·R A JanisE E Daniel

Citations

Aug 2, 1969·Nature·B E StatlandJ G White
Oct 29, 1968·Biochimica Et Biophysica Acta·S Matsushita, I D Raacke
Aug 1, 1971·Journal of Neurochemistry·I Diamond, A L Goldberg
Nov 1, 1968·British Journal of Pharmacology·W W Douglas
Jan 1, 1968·Progress in Biophysics and Molecular Biology·S Ebashi, M Endo
Feb 1, 1971·The Journal of General Physiology·Y N ChaK S Lee
Jun 15, 1966·Experientia·H J Schatzmann
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT
Mar 1, 1963·The Journal of General Physiology·A WEBERI REISS
Mar 25, 1960·Biochimica Et Biophysica Acta·H MUELLER

Related Concepts

Calcium [EPC]
Adenosine Diphosphate Measurement
Tissue Membrane
Magnesium Measurement
Oxalate
Calcium
Adenosine Triphosphatases
Oxalates
Uptake
Ouabain

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