Calcium/calmodulin-dependent protein kinase IIalpha mediates activation of mitogen-activated protein kinase and cytosolic phospholipase A2 in norepinephrine-induced arachidonic acid release in rabbit aortic smooth muscle cells.

The Journal of Biological Chemistry
M M MuthalifK U Malik

Abstract

We have investigated the contribution of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) and mitogen-activated protein kinase (MAP kinase) in norepinephrine (NE)-induced arachidonic acid (AA) release in rabbit aortic vascular smooth muscle cells (VSMC). NE enhanced release of AA via activation of cytosolic phospholipase A2 (cPLA2) but not secretory PLA2 in VSMC prelabeled with [3H]AA. NE (10 microM) enhanced CaM kinase II and MAP kinase activity. In cells transiently transfected with antisense oligonucleotides complementary to the translation initiation sites of CaM kinase II and MAP kinase, NE-induced AA release was inhibited by 100 and 35% respectively. Treatment of cells with PD-098059, a MAP kinase kinase inhibitor, or with MAP kinase antisense oligonucleotide reduced NE-induced activation of MAP kinase and cPLA2. NE-induced MAP kinase and cPLA2 activation was also inhibited in cells treated with a CaM kinase II inhibitor, KN-93, or with CaM kinase II antisense oligonucleotide. On the other hand, inhibition of MAP kinase kinase with PD-098059 or of MAP kinase with antisense oligonucleotides did not alter the NE-induced increase in CaM kinase II activity. Phosphorylation of MAP kinase and CaM kinase II by NE, stu...Continue Reading

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Citations

Apr 29, 1998·Molecular Neurobiology·K Fukunaga, E Miyamoto
Jan 20, 2004·European Journal of Pharmacology·Hiroyuki NakamuraToshihiko Murayama
Nov 28, 1997·Biochemical Pharmacology·S J Feinmark, J A Cornicelli
Jul 11, 2002·European Journal of Pharmacology·Yoshihumi KawanabeTomoh Masaki
May 16, 2001·Prostaglandins & Other Lipid Mediators·M M MuthalifK U Malik
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Sep 21, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Seyhan Sahan-FiratKafait U Malik
Jan 9, 2009·American Journal of Physiology. Renal Physiology·Frank ParkEllis D Avner
Jul 4, 2008·Journal of the American Society of Nephrology : JASN·Frank ParkEllis D Avner
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·M M MuthalifK U Malik
Sep 30, 2014·The Journal of Steroid Biochemistry and Molecular Biology·Maryam DoroudiZvi Schwartz
Jan 18, 2015·Neurobiology of Learning and Memory·Maria Grazia GiovanniniGiancarlo Pepeu
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