PMID: 6412227Sep 1, 1983Paper

Calcium/calmodulin inhibition of coupled NaCl transport in membrane vesicles from rabbit ileal brush border

Proceedings of the National Academy of Sciences of the United States of America
C C Fan, D W Powell

Abstract

The role of Ca2+ and calmodulin in regulating coupled NaCl transport has been investigated in membrane vesicles from rabbit ileal brush border. Uptake of 22Na+ and 36Cl- was determined by a rapid filtration technique in vesicles isolated with a sucrose density gradient ultracentrifugation method. Ca2+ on the inside of the vesicle inhibited Na+ uptake when Cl- was the anion and Cl- uptake when Na+ was the cation by approximately equal to 30%. Ca2+ on the outside had no effect. When gluconate was the anion or when choline was the cation, Na+ or Cl- uptake was reduced by only 9-12%. A similar inhibition of D-[3H]mannitol uptake (10-17%) suggests this was due to a nonspecific decrease in the membrane permeability. Other cations such as Ba2+ and Mg2+ had no effect, but La3+ inhibited Na+ and Cl- uptake to the same degree as Ca2+. Calmodulin (2 microM) in combination with Ca2+ (1 microM, free concentration) significantly inhibited Na+ uptake when Cl- was the anion by 21-32% and Cl- uptake when Na+ was the cation by 20-27%. This effect was completely reversed by 10 microM trifluoperazine. When gluconate was the anion or when choline was the cation, Na+ or Cl- uptake was unaffected by Ca2+/calmodulin and trifluoperazine. The Ki for Ca2...Continue Reading

References

Jul 26, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·S Grinstein, D Erlij
Apr 12, 1979·The Journal of Membrane Biology·I Schulz, K Heil
Jun 1, 1975·The Journal of General Physiology·R A FrizzellS G Schultz
Aug 1, 1980·The Journal of Clinical Investigation·M DonowitzG Pike
Jan 1, 1980·Annual Review of Biochemistry·C B KleeP G Richman
Jun 1, 1980·The Journal of Cell Biology·C L HoweT A Graves
Jan 1, 1982·Archives of Biochemistry and Biophysics·M LandtJ M McDonald

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Citations

Jan 1, 1986·Comparative Biochemistry and Physiology. A, Comparative Physiology·M Vernay
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K A AlbertP Greengard

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