CALCOCO2/NDP52 and SQSTM1/p62 differentially regulate coxsackievirus B3 propagation

Cell Death and Differentiation
Yasir MohamudHonglin Luo

Abstract

Cell autonomous immunity is the ability of individual cells to initiate a first line of host defense against invading microbes, such as viruses. Autophagy receptors, a diverse family of multivalent proteins, play a key role in this host response by detecting, sequestering, and eliminating virus in a process termed virophagy. To counteract this, positive-stranded RNA viruses, such as enteroviruses, have evolved strategies to circumvent the host autophagic machinery in an effort to promote viral propagation; however, the underlying mechanisms remain largely unclear. Here we studied the interaction between coxsackievirus B3 (CVB3) and the autophagy receptor SQSTM1 (sequestosome 1)/p62 and CALCOCO2/NDP52 (calcium binding and coiled-coil domain-containing protein 2/nuclear dot 10 protein 52). We demonstrated that SQSTM1 and CALCOCO2 differentially regulate CVB3 infection. We showed that knockdown of SQSTM1 causes increased viral protein production and elevated viral titers, whereas depletion of CALCOCO2 results in a significant inhibition of viral growth. Both receptors appear to have a role in virophagy through direct interaction with the viral capsid protein VP1 that undergoes ubiquitination during infection. Further investigation...Continue Reading

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Citations

Apr 24, 2020·Journal of Leukocyte Biology·Pak-Hin Hinson CheungDong-Yan Jin
Jun 17, 2020·Expert Opinion on Drug Discovery·Dasol KimHo Jeong Kwon
Nov 27, 2018·Virulence·Yasir Mohamud, Honglin Luo
May 29, 2020·Journal of Cell Science·Yingying CongFulvio Reggiori
Sep 29, 2020·International Journal of Chronic Obstructive Pulmonary Disease·Jian-Yu LiuYi-Qing Qu
Jan 24, 2020·Frontiers in Microbiology·Shuangqi FanJinding Chen
Mar 20, 2020·International Journal of Molecular Sciences·Shuangqi FanJinding Chen
Jun 20, 2019·Journal of Biomedical Science·Collins Oduor Owino, Justin Jang Hann Chu
Aug 30, 2019·Molecular Therapy Oncolytics·Haoyu DengHonglin Luo
Jan 16, 2021·Biochemical and Biophysical Research Communications·Yasir MohamudHonglin Luo
Mar 9, 2021·Trends in Microbiology·Christophe ViretMathias Faure
Mar 12, 2021·Autophagy·Yasir MohamudHonglin Luo
Mar 12, 2021·Journal of Cell Science·Jiamin YiHaixue Zheng
Jun 26, 2021·Cellular & Molecular Biology Letters·Päivi Ylä-Anttila
Jul 14, 2021·Scientific Reports·Michael F BodeNigel Mackman
Dec 4, 2021·IUBMB Life·Po-Yuan Ke

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Methods Mentioned

BETA
cleavage assay
Confocal Microscopy
PCR
co-immunoprecipitation
immunoprecipitation
pulldown
ubiquitination
Co-IP
ELISA
transfection

Software Mentioned

NIH ImageJ
ImageJ

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