Calculating the Degradation Rate of Individual Proteins Using Xenopus Extract Systems

Cold Spring Harbor Protocols
Gary S McDowell, Anna Philpott

Abstract

The Xenopus extract system has been used extensively as a simple, quick, and robust method for assessing the stability of proteins against proteasomal degradation. In this protocol, methods are provided for assessing the half-life of in vitro translated radiolabeled proteins using Xenopus egg or embryo extracts.

References

Jul 12, 2007·The Biochemical Journal·Jonathan M D VosperAnna Philpott
Sep 3, 2010·Biochemical and Biophysical Research Communications·Gary S McDowellAnna Philpott
Mar 6, 2012·Journal of Cell Science·Yelena Kravtsova-Ivantsiv, Aaron Ciechanover
Jun 5, 2013·The International Journal of Biochemistry & Cell Biology·Gary S McDowell, Anna Philpott
Jul 8, 2014·Biochemical and Biophysical Research Communications·Gary S McDowellAnna Philpott
Oct 21, 2016·The International Journal of Developmental Biology·Gary S McDowell, Anna Philpott
Feb 14, 2018·Cold Spring Harbor Protocols·Matthew C Good, Rebecca Heald

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Citations

Jun 4, 2020·Annals of the New York Academy of Sciences·Chi Him PoonLee Wei Lim
Jul 22, 2020·Annual Review of Microbiology·Sven J Saupe
Jul 8, 2018·Cold Spring Harbor Protocols·Romain Gibeaux, Rebecca Heald
May 18, 2018·Cold Spring Harbor Protocols·Gary S McDowell, Anna Philpott

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