Abstract
The translational friction coefficients and intrinsic viscosities of four proteins (ribonuclease A, lysozyme, myoglobin, and chymotrypsinogen A) are calculated using atomic-level structural details. Inclusion of a 0.9-A-thick hydration shell allows calculated results for both hydrodynamic properties of each protein to reproduce experimental data. The use of detailed protein structures is made possible by relating translational friction and intrinsic viscosity to capacitance and polarizability, which can be calculated easily. The 0.9-A hydration shell corresponds to a hydration level of 0.3-0.4 g water/g protein. Hydration levels within this narrow range are also found by a number of other techniques such as nuclear magnetic resonance spectroscopy, infrared spectroscopy, calorimetry, and computer simulation. The use of detailed protein structures in predicting hydrodynamic properties thus allows hydrodynamic measurement to join the other techniques in leading to a unified picture of protein hydration. In contrast, earlier interpretations of hydrodynamic data based on modeling proteins as ellipsoids gave hydration levels that varied widely from protein to protein and thus challenged the existence of a unified picture of protein h...Continue Reading
References
Aug 1, 1979·Archives of Biochemistry and Biophysics·P G Squire, M E Himmel
Apr 19, 1988·Biochemistry·A WlodawerG L Gilliland
Jun 1, 1988·Biopolymers·R M Venable, R W Pastor
Jan 25, 1974·Journal of Molecular Biology·R Diamond
Jan 27, 1971·Journal of the American Chemical Society·I D Kuntz
Nov 1, 1968·Archives of Biochemistry and Biophysics·H B Bull, K Breese
Jun 1, 1995·Biophysical Journal·S A Allison, V T Tran
Aug 1, 1993·Biophysical Journal·H X Zhou
Oct 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·P J Steinbach, B R Brooks
Dec 1, 1995·Biophysical Journal·J Norberg, L Nilsson
Citations
Aug 1, 2006·Journal of Colloid and Interface Science·Luciano CaseliOsvaldo N Oliveira
Jan 1, 1997·Progress in Biophysics and Molecular Biology·S E Harding
Jan 24, 2002·Biophysical Chemistry·H Durchschlag, P Zipper
Jan 24, 2002·Biophysical Chemistry·J García de la Torre
Jan 24, 2002·Biophysical Chemistry·H X Zhou
May 8, 1999·Biophysical Journal·S E HardingD J Winzor
Oct 26, 2005·Biophysical Chemistry·Mikulás Bánó, Jozef Marek
Feb 13, 2007·Structure·Ramzi Alsallaq, Huan-Xiang Zhou
May 23, 2006·Biophysical Journal·Sergio Aragon, David K Hahn
Aug 17, 2011·Biophysical Journal·A OrtegaJ García de la Torre
Dec 10, 1999·Biophysical Journal·B CarrascoS E Harding
Sep 6, 2011·Biophysical Journal·Xiaodong PangHuan-Xiang Zhou
Feb 2, 2000·Biophysical Journal·J García De La TorreB Carrasco
Apr 11, 1997·Journal of Molecular Biology·H X Zhou, M Vijayakumar
Jun 20, 1998·Journal of Molecular Biology·M VijayakumarH X Zhou
Jan 5, 2000·Biophysical Journal·E BanachowiczA Patkowski
Oct 13, 2007·Proteins·Ramzi Alsallaq, Huan-Xiang Zhou
Jun 18, 2005·Biopolymers·Subhajyoti DeSwagata Dasgupta
Aug 14, 2008·The Journal of Biological Chemistry·Olivier DelelisEric Deprez
Aug 17, 2019·Scientific Reports·Gareth ShannonPhilip M Williams
Apr 22, 1997·Biophysical Chemistry·W S PriceY Arata
May 17, 2005·Structure·Nithin RaiMattia Rocco
Nov 11, 2020·Molecular Pharmaceutics·Mahlet A WoldeyesChristopher J Roberts
Mar 21, 2015·The Journal of Physical Chemistry. B·Jian DaiHuan-Xiang Zhou
May 27, 2008·Journal of Colloid and Interface Science·José A Fornés