Calculation of translational friction and intrinsic viscosity. II. Application to globular proteins

Biophysical Journal
X Z Zhou

Abstract

The translational friction coefficients and intrinsic viscosities of four proteins (ribonuclease A, lysozyme, myoglobin, and chymotrypsinogen A) are calculated using atomic-level structural details. Inclusion of a 0.9-A-thick hydration shell allows calculated results for both hydrodynamic properties of each protein to reproduce experimental data. The use of detailed protein structures is made possible by relating translational friction and intrinsic viscosity to capacitance and polarizability, which can be calculated easily. The 0.9-A hydration shell corresponds to a hydration level of 0.3-0.4 g water/g protein. Hydration levels within this narrow range are also found by a number of other techniques such as nuclear magnetic resonance spectroscopy, infrared spectroscopy, calorimetry, and computer simulation. The use of detailed protein structures in predicting hydrodynamic properties thus allows hydrodynamic measurement to join the other techniques in leading to a unified picture of protein hydration. In contrast, earlier interpretations of hydrodynamic data based on modeling proteins as ellipsoids gave hydration levels that varied widely from protein to protein and thus challenged the existence of a unified picture of protein h...Continue Reading

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Citations

Aug 1, 2006·Journal of Colloid and Interface Science·Luciano CaseliOsvaldo N Oliveira
Oct 26, 2005·Biophysical Chemistry·Mikulás Bánó, Jozef Marek
Feb 13, 2007·Structure·Ramzi Alsallaq, Huan-Xiang Zhou
Sep 6, 2011·Biophysical Journal·Xiaodong PangHuan-Xiang Zhou
Feb 2, 2000·Biophysical Journal·J García De La TorreB Carrasco
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Jan 5, 2000·Biophysical Journal·E BanachowiczA Patkowski
Jun 18, 2005·Biopolymers·Subhajyoti DeSwagata Dasgupta
Aug 17, 2019·Scientific Reports·Gareth ShannonPhilip M Williams
Mar 21, 2015·The Journal of Physical Chemistry. B·Jian DaiHuan-Xiang Zhou
May 27, 2008·Journal of Colloid and Interface Science·José A Fornés

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