CalFitter: a web server for analysis of protein thermal denaturation data

Nucleic Acids Research
Stanislav MazurenkoJiri Damborsky

Abstract

Despite significant advances in the understanding of protein structure-function relationships, revealing protein folding pathways still poses a challenge due to a limited number of relevant experimental tools. Widely-used experimental techniques, such as calorimetry or spectroscopy, critically depend on a proper data analysis. Currently, there are only separate data analysis tools available for each type of experiment with a limited model selection. To address this problem, we have developed the CalFitter web server to be a unified platform for comprehensive data fitting and analysis of protein thermal denaturation data. The server allows simultaneous global data fitting using any combination of input data types and offers 12 protein unfolding pathway models for selection, including irreversible transitions often missing from other tools. The data fitting produces optimal parameter values, their confidence intervals, and statistical information to define unfolding pathways. The server provides an interactive and easy-to-use interface that allows users to directly analyse input datasets and simulate modelled output based on the model parameters. CalFitter web server is available free at https://loschmidt.chemi.muni.cz/calfitter/.

References

Jul 27, 2004·Protein Science : a Publication of the Protein Society·Mark E HarderMichael I Schimerlik
Oct 17, 2008·Computer Methods and Programs in Biomedicine·Piotr M SzczypińskiArtur Klepaczko
Jan 22, 2009·Analytical Biochemistry·Kenneth A JohnsonThomas Blom
May 24, 2014·Nature Reviews. Molecular Cell Biology·Tuomas P J KnowlesChristopher M Dobson
Dec 27, 2017·Biotechnology and Bioengineering·Pavel DvorakJiri Damborsky

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Citations

Nov 20, 2018·Current Protein & Peptide Science·Rajeshwari Sinha, Pratyoosh Shukla
Dec 11, 2020·Nature·Georg K A HochbergJoseph W Thornton
May 19, 2021·Chembiochem : a European Journal of Chemical Biology·Yong Jia Bu, Mark Nitz
Jun 18, 2021·Biochimica Et Biophysica Acta. General Subjects·Veronica S ValadaresAdolfo H Moraes
Sep 28, 2021·Biophysical Journal·Victor Y ZhaoEugene I Shakhnovich

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