CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane of epithelial cells including taste cells

Scientific Reports
Makiko KashioAkiyuki Taruno

Abstract

The CALHM1/CALHM3 channel in the basolateral membrane of polarized taste cells mediates neurotransmitter release. However, mechanisms regulating its localization remain unexplored. Here, we identified CALHM1/CALHM3 in the basolateral membrane of type II taste cells in discrete puncta localized close to afferent nerve fibers. As in taste cells, CALHM1/CALHM3 was present in the basolateral membrane of model epithelia, although it was distributed throughout the membrane and did not show accumulation in puncta. We identified canonical basolateral sorting signals in CALHM1 and CALHM3: tyrosine-based and dileucine motifs. However, basolateral sorting remained intact in mutated channels lacking those signals, suggesting that non-canonical signals reside elsewhere. Our study demonstrates intrinsic basolateral sorting of CALHM channels in polarized cells, and provides mechanistic insights.

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Citations

Jan 9, 2020·The European Journal of Neuroscience·Masafumi IwamotoYoshitaka Ohtubo
Sep 17, 2020·Pflügers Archiv : European journal of physiology·Akiyuki TarunoJ Kevin Foskett
Aug 25, 2020·Science Advances·Yue RenYuequan Shen
Oct 20, 2020·The Journal of General Physiology·Pablo S GaeteJorge E Contreras
Nov 12, 2020·Experimental Physiology·Keita TakeuchiYoshitaka Ohtubo
Jan 2, 2021·Trends in Endocrinology and Metabolism : TEM·Christina E May, Monica Dus
Feb 23, 2021·Current Opinion in Physiology·Thomas E Finger, Linda A Barlow
Apr 1, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Motoki OkuiAkiyuki Taruno
Apr 10, 2021·The Journal of General Physiology·Carsten MimGerhard Dahl
Jun 6, 2021·Molecular and Cellular Biochemistry·Eijaz Ahmed BhatJohra Khan

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Methods Mentioned

BETA
transfection
Fluorescence
pull-down
ubiquitination
PCR

Software Mentioned

Zeiss Zen Blue
CALHM3
Image J
Cter

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