PMID: 8941557Nov 1, 1996Paper

Calibration of Pd-porphyrin phosphorescence for oxygen concentration measurements in vivo

Journal of Applied Physiology
M Sinaasappel, C Ince

Abstract

Quantitative measurement of oxygen concentrations in the microvasculature is of prime importance in issues related to oxygen transport to tissue. The introduction of the quenching of the Pd-porphyrin phosphorescence as oxygen sensor in vivo by Wilson et al. (J. Appl. Physiol. 74: 580-589, 1993) has provided in this context a major advance in this area of research. For in vivo application, the dye is coupled to albumin to restrict the dye to the circulation and to measure oxygen in the physiological range. In this study a phosphorimeter with a gated photomultiplier is presented and validated. Furthermore, a nonlinear-fit method using the Marquardt-Levenberg algorithm is used to calculate the decay time. With this new phosphorimeter, calibration measurements were performed to investigate the effects of pH, temperature, and diffusivity. The results present a preparation method for albumin coupling of the dye that eliminates the pH dependency of the quenching kinetics. Furthermore, the decreased oxygen diffusivity of serum was compared with that of water, and it was shown that calibration constants measured in water can be extrapolated to serum.

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Citations

Jun 24, 2010·Chemical Society Reviews·Peter R Ogilby
Oct 3, 2012·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Roland N Pittman
Feb 14, 2014·Photochemistry and Photobiology·Mark A Weston, Michael S Patterson
Jun 13, 2015·Journal of Applied Physiology·Can Ince, Egbert G Mik
Jul 20, 2002·Photochemistry and Photobiology·Egbert G MikCan Ince
May 24, 2007·Journal of Fluorescence·Yuezhi LiHuiling Yang
Jan 11, 2008·American Journal of Physiology. Renal Physiology·Egbert G MikCan Ince
Jun 5, 2008·Journal of the Royal Society, Interface·Joe DragavonLloyd Burgess
Feb 6, 2016·Journal of Applied Physiology·Aleksander S Golub, Roland N Pittman
Dec 10, 2016·Journal of Applied Physiology·Philippe GuerciCan Ince
Jun 11, 1999·The American Journal of Physiology·M SinaasappelC Ince
Aug 14, 1999·Critical Care Medicine·C Ince, M Sinaasappel
Oct 12, 2004·Journal of Applied Physiology·Lothar A SchwarteCan Ince
Jun 21, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Aleksander S GolubRoland N Pittman
Oct 15, 2010·Biotechnology Progress·Angela Guaccio, Paolo A Netti
Aug 11, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alfonso Blázquez-CastroPeter R Ogilby
Sep 21, 2013·Physics in Medicine and Biology·Mark A Weston, Michael S Patterson
Jul 5, 2012·The Keio Journal of Medicine·Nobuhiko SasakiHaruyuki Minamitani
Mar 2, 2011·Current Opinion in Critical Care·Alex Dyson, Mervyn Singer
May 8, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Aleksander S GolubRoland N Pittman
Dec 21, 2016·Critical Care : the Official Journal of the Critical Care Forum·Lara ZafraniCan Ince
Sep 25, 2003·Journal of the American Chemical Society·Dongshan ZhouGi Xue
Jun 14, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Aleksander S Golub, Roland N Pittman
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Jun 26, 1998·Journal of Photochemistry and Photobiology. B, Biology·B W McIlroyA J MacRobert
Aug 28, 2003·Biosensors & Bioelectronics·Kosuke TsukadaHaruyuki Minamitani

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