Calibration standards for multicenter clinical trials of fluorescence spectroscopy for in vivo diagnosis

Journal of Biomedical Optics
Nena M MarínRebecca Richards-Kortum

Abstract

In the context of clinical trials, calibration protocols for optical instruments that ensure measurement accuracy and the ability to carry out meaningful comparisons of data acquired from multiple instruments are required. A series of calibration standards and procedures are presented to assess technical feasibility of optical devices for cervical precancer detection. Measurements of positive and negative standards, and tissue are made with two generations of research grade spectrometers. Calibration accuracy, ability of standards to correct and account for changes in experimental conditions, and device components are analyzed. The relative frequency of measured calibration standards is investigated retrospectively using statistical analysis of trends in instrument performance. Fluorescence measurements of standards and tissue made with completely different spectrometers show good agreement in intensity and lineshape. Frequency of wavelength calibration standards is increased to every 2 h to compensate for thermal drifts in grating mount. Variations in illumination energy detected between standards and patient measurements require probe redesign to allow for simultaneous acquisition of illumination power with every patient meas...Continue Reading

References

Jul 1, 1992·Academic Medicine : Journal of the Association of American Medical Colleges·B Littenberg
Jan 1, 1994·Gynecologic Oncology·N RamanujamR Richards-Kortum
Nov 24, 1998·Photochemistry and Photobiology·G A WagnièresB C Wilson
Mar 21, 2002·American Journal of Obstetrics and Gynecology·Irene GeorgakoudiMichael S Feld
Oct 11, 2002·IEEE Transactions on Bio-medical Engineering·Sung K ChangRebecca Richards-Kortum
Jul 23, 2003·Photochemistry and Photobiology·Erin M GillNirmala Ramanujam
May 29, 2004·American Journal of Obstetrics and Gynecology·Warner K HuhRonald D Alvarez
Jan 1, 2001·Journal of Research of the National Institute of Standards and Technology·A K GaigalasA Schwartz

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Citations

Apr 16, 2010·Journal of Fluorescence·Christian WürthUte Resch-Genger
Jan 7, 2009·Journal of Biomedical Optics·Crystal Redden WeberRebecca Richards-Kortum
Mar 10, 2009·Current Opinion in Biotechnology·J Quincy BrownNirmala Ramanujam
Sep 11, 2010·International Journal of Cancer. Journal International Du Cancer·Scott B CantorMichele Follen
Jan 25, 2011·Biomedical Optics Express·José-Miguel YamalMichele Follen
Apr 2, 2014·Biomedical Optics Express·Bing YuDaron G Ferris
May 23, 2008·Analytical Chemistry·Mark LowryIsiah M Warner

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